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Cellular determinants of neuronal plasticity
on the level of single synapses in vivo

Objective

The search for the fundamental mechanisms of learning and experience-dependent memory formation in the brain has long been a central quest in neuroscience. The neocortex is a particularly relevant region for plasticity because it is involved in sensory, motor, and cognitive tasks with strong learning components. However, despite many years of intensive research our knowledge of the neuronal mechanisms of plasticity on the level of single synapses in the intact living brain is still very limited.
Here I propose the use of cutting edge technology, including the ultrasensitive LOTOS procedure of in vivo two-photon calcium imaging that was developed in our laboratory, to investigate for the first time the functional properties and the plasticity of signal synapses in auditory cortical pyramidal neurons of layers 2/3, 4 and 5 in vivo. For the study of the cellular determinants of synaptic plasticity we will focus on an associative learning paradigm underlying cued fear conditioning. Importantly, this paradigm can be rapidly and effectively induced not only in awake, but also in anesthetized animals and is therefore ideally suited for these studies. In addition to a comprehensive analysis of wild type animals, we will perform experiments in mouse models of Alzheimer’s disease (AD), aiming to identify the cellular cause of the devastating impairment of memory formation observed in patients suffering from AD.

Call for proposal

ERC-2012-ADG_20120314
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Funding Scheme

ERC-AG - ERC Advanced Grant

Host institution

TECHNISCHE UNIVERSITAET MUENCHEN
Address
Arcisstrasse 21
80333 Muenchen
Germany
Activity type
Higher or Secondary Education Establishments
EU contribution
€ 2 404 800
Principal investigator
Arthur Konnerth (Prof.)
Administrative Contact
Ulrike Ronchetti (Ms.)

Beneficiaries (1)

TECHNISCHE UNIVERSITAET MUENCHEN
Germany
EU contribution
€ 2 404 800
Address
Arcisstrasse 21
80333 Muenchen
Activity type
Higher or Secondary Education Establishments
Principal investigator
Arthur Konnerth (Prof.)
Administrative Contact
Ulrike Ronchetti (Ms.)