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Assembly and function of the chromosome periphery during mitosis

Obiettivo

While chromosomes fill the nuclear space as decondensed chromosome territories during interphase, they are dramatically re-structured into compact rod-shaped rigid bodies during mitosis. The aim of my work is to elucidate the molecular mechanisms underlying this cell cycle-dependent chromosome reorganization. Unlike previous studies, I plan to focus on a yet poorly characterized chromosomal domain defined as ‘chromosome periphery’. I first plan to establish a detailed map of the proteins that target to the chromosome periphery in human cells, applying both high-resolution three-dimensional microscopy and superresolution fluorescence imaging techniques as well as molecular biology techniques, including chromatin immunoprecipitation combined with next generation sequencing (ChIP-seq) analysis. The combination of these approaches will provide me with information on different scales, first at the cell biological level and second at the DNA sequence level, to obtain a complete picture of the localization of the proteins that may organize the chromosome periphery. I will then investigate the functional relevance of chromosome periphery components in mitotic chromosome morphogenesis by RNAi screening in tissue culture cells. I expect that this work will provide insight into how a protein network sets up a chromosomal domain and how such a domain can contribute to the formation of mitotic chromosomes.

Invito a presentare proposte

FP7-PEOPLE-2012-IEF
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Coordinatore

INSTITUT FUER MOLEKULARE BIOTECHNOLOGIE GMBH
Contributo UE
€ 179 137,20
Indirizzo
DR BOHRGASSE 3
1030 Wien
Austria

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Regione
Ostösterreich Wien Wien
Tipo di attività
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Contatto amministrativo
Tanja Winkler (Mrs.)
Collegamenti
Costo totale
Nessun dato