Fluorescent macrocycles as functional viscosity probes in live cells

Projektinformationen

FLUMVI

ID Finanzhilfevereinbarung: 327334

Abgeschlossenes Projekt

Startdatum 1 Mai 2013

Enddatum 26 Mai 2016

Finanziert unter

• Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)

Gesamtkosten € 309 235,20

EU-Beitrag € 309 235,20

Koordiniert durch

IMPERIAL COLLEGE OF SCIENCE TECHNOLOGY AND MEDICINE
United Kingdom

Ziel

The present proposal aims to develop two novel classes of functional probes, which will enable the measurement of viscosity in cells. This important physiological parameter will be measured during normal cell function and during photodynamic therapy (PDT), a photoactivated cancer treatment of clinical importance. To achieve these objectives, we will characterise and use two types of tetrapyrrole macrocycles: porphycenes and porphyrazines as red/NIR emissive molecular rotors and PDT sensitizers and we will measure viscosity based on their fluorescence lifetime. The molecules will be used first in model systems such as vesicles, liposomes and gels and then in the complex environment of living cells. Protocols for the simultaneous detection of viscosity and other important physiological parameters such as pH will be developed. The novel methods will allow the quantification of viscosity in heterogeneous media, which is fundamental for understanding the complex dynamics of important biological, chemical and physical processes.

Wissenschaftliches Gebiet

• natural sciences › physical sciences › optics › microscopy › fluorescence lifetime imaging
• medical and health sciences › clinical medicine › oncology

Programm/Programme

• FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)

Thema/Themen

• FP7-PEOPLE-2012-IEF - Marie-Curie Action: "Intra-European fellowships for career development"

Aufforderung zur Vorschlagseinreichung

FP7-PEOPLE-2012-IEF
Andere Projekte für diesen Aufruf anzeigen

Finanzierungsplan

MC-IEF - Intra-European Fellowships (IEF)

Koordinator

IMPERIAL COLLEGE OF SCIENCE TECHNOLOGY AND MEDICINE

Kontakt Verwaltung

Brooke Alasya (Ms.)

• EU-Beitrag: € 309 235,20
• Adresse: SOUTH KENSINGTON CAMPUS EXHIBITION ROAD ; SW7 2AZ LONDON ; Vereinigtes Königreich
• Region: London › Inner London — West › Westminster
• Aktivitätstyp: Higher or Secondary Education Establishments
• Gesamtkosten: Keine Daten