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An integrated multidisciplinary tissue engineering approach combining novel high-throughput screening and advanced methodologies to create complex biomaterials-stem cells constructs

An integrated multidisciplinary tissue engineering approach combining novel high-throughput screening and advanced methodologies to create complex biomaterials-stem cells constructs

Objective

New developments on tissue engineering strategies should realize the complexity of tissue remodelling and the inter-dependency of many variables associated to stem cells and biomaterials interactions. ComplexiTE proposes an integrated approach to address such multiple factors in which different innovative methodologies are implemented, aiming at developing tissue-like substitutes with enhanced in vivo functionality. Several ground-breaking advances are expected to be achieved, including: i) improved methodologies for isolation and expansion of sub-populations of stem cells derived from not so explored sources such as adipose tissue and amniotic fluid; ii) radically new methods to monitor human stem cells behaviour in vivo; iii) new macromolecules isolated from renewable resources, especially from marine origin; iv) combinations of liquid volumes mingling biomaterials and distinct stem cells, generating hydrogel beads upon adequate cross-linking reactions; v) optimised culture of the produced beads in adequate 3D bioreactors and a novel selection method to sort the beads that show a (pre-defined) positive biological reading; vi) random 3D arrays validated by identifying the natural polymers and cells composing the positive beads; v) 2D arrays of selected hydrogel spots for brand new in vivo tests, in which each spot of the implanted chip may be evaluated within the living animal using adequate imaging methods; vi) new porous scaffolds of the best combinations formed by particles agglomeration or fiber-based rapid-prototyping. The ultimate goal of this proposal is to develop breakthrough research specifically focused on the above mentioned key issues and radically innovative approaches to produce and scale-up new tissue engineering strategies that are both industrially and clinically relevant, by mastering the inherent complexity associated to the correct selection among a great number of combinations of possible biomaterials, stem cells and culturing conditions.

Principal Investigator

Rui Luis Gonçalves Dos Reis (Prof.)

Host institution

UNIVERSIDADE DO MINHO

Address

Largo Do Paco
4704 553 Braga

Portugal

Activity type

Higher or Secondary Education Establishments

EU Contribution

€ 2 320 000

Principal Investigator

Rui Luis Gonçalves Dos Reis (Prof.)

Administrative Contact

Rui Luis Gonçalves Dos Reis (Prof.)

Beneficiaries (1)

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UNIVERSIDADE DO MINHO

Portugal

EU Contribution

€ 2 320 000

Project information

Grant agreement ID: 321266

Status

Closed project

  • Start date

    1 May 2013

  • End date

    30 April 2018

Funded under:

FP7-IDEAS-ERC

  • Overall budget:

    € 2 320 000

  • EU contribution

    € 2 320 000

Hosted by:

UNIVERSIDADE DO MINHO

Portugal