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Ultra High-throughput platform for the selection of thermostable proteins by thermophilic in vitro transcription-translation and microfluidics

Objective

"This initiative connects four companies and four academic groups in pursuit of a central biotechnological challenge: to identify and improve enzymes for application in industrial biocatalysis. The technology interchange is centered around key technologies to generate thermostabilized proteins. We will develop, share and spread the following approaches (i) folding interference assays with a thermostable fluorescent protein reporter, (ii) high throughput assays in microfluidic droplets that allow screening of libraries on the order of 10e8, (iii) in vitro expression in microdroplets using expression extracts from themophiles, and (iv) approaches to generate enzyme libraries from metagenomic sources and by randomization of existing proteins. Suitable assays will be established for a list of target enzymes of interest to the industrial partners (most prominently hydrolases – such as esterases, cellulases and proteases), ensuring wide applicability and industrial relevance. Improved mutants will be characterized biochemically and scale-up and optimization for protein production by fermentation techniques will be attempted. An experienced partner who will enable others by workshop training, interchange of technical and academic personnel and joint projects represents each of these approaches. The objective of this application is to make the considerable technical skill involved in molecular biology, enzymology and screening technologies available to a wider circle of users, ranging from academic labs to very recent spin-off companies and the world’s largest provider of industrial enzymes."

Call for proposal

FP7-PEOPLE-2012-IAPP
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Coordinator

UNIVERSIDAD AUTONOMA DE MADRID
Address
Calle Einstein 3 Ciudad Univ Cantoblanco Rectorado
28049 Madrid
Spain

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Activity type
Higher or Secondary Education Establishments
Administrative Contact
Mª Carmen Puerta (Ms.)
EU contribution
€ 372 262,28

Participants (7)

THE CHANCELLOR MASTERS AND SCHOLARS OF THE UNIVERSITY OF CAMBRIDGE
United Kingdom
EU contribution
€ 437 143,53
Address
Trinity Lane The Old Schools
CB2 1TN Cambridge

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Activity type
Higher or Secondary Education Establishments
Administrative Contact
Renata Schaeffer (Ms.)
UNIVERSIDADE DA CORUNA
Spain
EU contribution
€ 143 949,48
Address
Calle De La Maestranza 9
15001 La Coruna

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Activity type
Higher or Secondary Education Establishments
Administrative Contact
María Isabel González Siso (Dr.)
UNIVERSIDAD DE VIGO
Spain
EU contribution
€ 117 397,46
Address
Lg Campus Lagoas Marcosende
36310 Vigo Pontevedra

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Activity type
Higher or Secondary Education Establishments
Administrative Contact
Anxo Moreira (Mr.)
BIOCHEMIZE SL
Spain
EU contribution
€ 112 859,12
Address
Carrer Marie Curie 8
08005 Barcelona

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Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Jaume Mir (Dr.)
DROP-TECH LTD
United Kingdom
EU contribution
€ 330 341,44
Address
Canterbury Street, Canterbury Court 91
CB4 3QU Cambridge

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Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Liisa Van Vliet (Dr.)
GALCHIMIA SA
Spain
EU contribution
€ 172 542,72
Address
Parque Empresarial De Touro Parcela 26-27 Fonte Diaz
15822 Touro A Coruna

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Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
María José Varela (Ms.)
NOVOZYMES A/S
Denmark
EU contribution
€ 287 301,70
Address
Krogshoejvej 36
2880 Bagsvaerd

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Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Ali Fallah Araghi (Dr.)