Objectif Considerable focus has been placed on the influence of endogenous and exogenous interfering RNA on stem cell differentiation and behavior and substantial effort to elucidate the potential of reprogramming stem cells to guide of them towards a cardiomyogenic phenotype as a therapeutic option for heart disease. The majority of studies employ gain- or loss- of function experimental models or perform global microArrays to determine microRNAs that exhibit candidacy as governing dictators of differentiation. Delivery of these potentially therapeutic biomolecules can be achieved through viral vectors; which raises concerns of mutagenesis and immunogenicity, or their less efficient counterparts – non-viral vectors.The fellow presents here, the use of femtosecond laser Optotransfection to create transient nanopores in the cell membrane to allow entry of exogenous microRNAs efficiently into the cell with minimal effect on cellular viability. The fellow aims to apply this system in an automated process, combining robotics, software engineering and management of data in a multi-disciplinary project toward which he will add his expert knowledge in microRNA delivery in vitro, cardiac related microRNAs, in vitro cell culture and biomedical engineering. This project places the fellow in a unique position to apply biomedical research technology to an automated process to yield high-throughput standardized experiments which could yield potent formulations of microRNAs to reprogram stem cells towards a cardiac progenitor cell lineage.Herein, this project proposal describes the multidisciplinary nature of this project, the execution and management of this project by the fellow and the multiple collaborators that will each equip the fellow with cutting-edge research skills. The goals of the fellow’s project will place specific focus to ensure that the training of the fellow brings him to a level of competitive scientific excellence both in Europe and Internationally. Champ scientifique natural sciencesbiological sciencesbiochemistrybiomoleculesmedical and health sciencesmedical biotechnologycells technologiesstem cellsnatural sciencesbiological sciencesgeneticsRNAengineering and technologyelectrical engineering, electronic engineering, information engineeringelectronic engineeringroboticsnatural sciencesphysical sciencesopticslaser physics Programme(s) FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Thème(s) FP7-PEOPLE-2012-IEF - Marie-Curie Action: "Intra-European fellowships for career development" Appel à propositions FP7-PEOPLE-2012-IEF Voir d’autres projets de cet appel Régime de financement MC-IEF - Intra-European Fellowships (IEF) Coordinateur FRAUNHOFER GESELLSCHAFT ZUR FORDERUNG DER ANGEWANDTEN FORSCHUNG EV Contribution de l’UE € 168 794,40 Adresse HANSASTRASSE 27C 80686 Munchen Allemagne Voir sur la carte Région Bayern Oberbayern München, Kreisfreie Stadt Type d’activité Research Organisations Contact administratif Christoph Schulte (Mr.) Liens Contacter l’organisation Opens in new window Site web Opens in new window Coût total Aucune donnée
