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Development of chemical biology tools for the elucidation of protein phosphatase-1 substrates and druggability

Objectif

Protein serine/threonine phosphatases (PSTPs) are considered undruggable although they are involved in the most prominent post-translational modifications. This is mainly due to an apparent lack of substrate specificity. One important PSTP is protein phosphatase-1 (PP1), a ubiquitous PSTP that is predicted to catalyze about 1/3rd of Ser and Thr dephosphorylations in eukaryotic cells, counteracting hundreds of kinases. PP1 has broad substrate specificity but is restrained in vivo by numerous PP1-interacting proteins functioning for example as substrate-targeting proteins and forming specific holoenzymes with PP1. PP1 holoenzymes play a role in many different diseases such as cancer (counteracting oncogenic kinases), diabetes (insulin release), Alzheimer’s (dephosphorylation of Tau protein) and HIV (viral translation). Currently, there are no chemical modulators available that target PP1 selectively, except that we recently developed the first compound that selectively activates PP1 in intact cells, leading to rapid dephosphorylation of PP1 substrates. The activator does not act on the most closely related protein phosphatase-2A. This proposal aims to generate and apply tools for the investigation of PP1, in part based on our previously developed activator. The tools include selective, photo- and enzymatically releasable chemical inhibitors and activators and semisynthetic proteins, and they will be applied to study PP1–substrate interactions and help identify the correlating interacting proteins. The proposed research will provide long-sought selective chemical tools to study PP1 by applying new concepts of activator and inhibitor design using peptide and small molecule chemistry to an enzyme class that is difficult to be targeted chemically. This research program will contribute to a much more detailed understanding of PP1 biology, and will open doors to investigate PP1 and its holoenzymes as drug targets.

Appel à propositions

ERC-2013-StG
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Régime de financement

ERC-SG - ERC Starting Grant

Institution d’accueil

ALBERT-LUDWIGS-UNIVERSITAET FREIBURG
Contribution de l’UE
€ 488 332,76
Adresse
FAHNENBERGPLATZ
79098 Freiburg
Allemagne

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Région
Baden-Württemberg Freiburg Freiburg im Breisgau, Stadtkreis
Type d’activité
Higher or Secondary Education Establishments
Chercheur principal
Maja Banks-Köhn (Prof.)
Contact administratif
Christian Jäger (Mr.)
Liens
Coût total
Aucune donnée

Bénéficiaires (2)