Objetivo "Large proportions of eukaryotic genomes consist of transposable elements (TEs), predominantly of retrotransposons. They are generally considered as intra-chromosomal parasites. However, their periodical activity bursts influenced organization of host genomes and contributed to beneficial traits. Remarkably, number of transposon generated phenotypic innovations was subsequently selected by humans during plant domestication and breeding. Therefore, retrotransposons could be also considered as attractive endogenous source of genetic variation. Unfortunately so far there is no experimental/technological means to exploit this potential in a controlled fashion.Recently my laboratory revealed surprisingly selective epigenetic, environmental and developmental mechanisms controlling retrotransposition in Arabidopsis. Here, I intend to build on this knowledge and establish well controlled retrotransposition system a crop plant to liberate its innate genetic diversity buried in silenced TEs.To achieve this, I propose a stepwise strategy with the following three objectives:1. Deeper understanding of molecular mechanisms and environmental cues controlling retrotransposition in Arabidopsis thaliana and use this model plant to develop universal methodology for detection of retrotransposition.2. Transfer the knowledge and methodologies to non model plant. I propose to use Arabidopsis lyrata as close relative of A. thaliana, however with 50% enlarged genome due to expansion of retrotransposon populations.3. Using methodologies and experimental conditions developed for A. thaliana and A. lyrata we will apply them to maize where we can also take advantage of genetic resources (mutants in epigenetic regulation) in conjunction with responses to selected abiotic stresses.If the progress towards above listed objectives was swift, I will initiate collaboration with a corn breeding company to create EVOBREED generated population for evaluation of its phenotypic diversity." Ámbito científico agricultural sciencesagriculture, forestry, and fisheriesagricultureagronomyplant breedingnatural sciencesbiological sciencesgeneticsepigeneticsnatural sciencesbiological sciencesgeneticsgenomeseukaryotic genomes Programa(s) FP7-IDEAS-ERC - Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Tema(s) ERC-AG-LS9 - ERC Advanced Grant - Applied life sciences and biotechnology Convocatoria de propuestas ERC-2012-ADG_20120314 Consulte otros proyectos de esta convocatoria Régimen de financiación ERC-AG - ERC Advanced Grant Institución de acogida THE CHANCELLOR MASTERS AND SCHOLARS OF THE UNIVERSITY OF CAMBRIDGE Aportación de la UE € 2 497 062,00 Dirección TRINITY LANE THE OLD SCHOOLS CB2 1TN Cambridge Reino Unido Ver en el mapa Región East of England East Anglia Cambridgeshire CC Tipo de actividad Higher or Secondary Education Establishments Investigador principal Jerzy Paszkowski (Prof.) Contacto administrativo Renata Schaeffer (Ms.) Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Coste total Sin datos Beneficiarios (1) Ordenar alfabéticamente Ordenar por aportación de la UE Ampliar todo Contraer todo THE CHANCELLOR MASTERS AND SCHOLARS OF THE UNIVERSITY OF CAMBRIDGE Reino Unido Aportación de la UE € 2 497 062,00 Dirección TRINITY LANE THE OLD SCHOOLS CB2 1TN Cambridge Ver en el mapa Región East of England East Anglia Cambridgeshire CC Tipo de actividad Higher or Secondary Education Establishments Investigador principal Jerzy Paszkowski (Prof.) Contacto administrativo Renata Schaeffer (Ms.) Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Coste total Sin datos