Objectif "Recently through de novo peptide design, we have discovered and presented a new protein structure. This is an all-parallel, 6-helix bundle with a continuous central channel of 0.5 – 0.6 nm diameter. We posit that this is one of a broader class of protein structures that we call the alpha-helical barrels. Here, in three Work Packages, we propose to explore these structures and to develop protein functions within them. First, through a combination of computer-aided design, peptide synthesis and thorough biophysical characterization, we will examine the extents and limits of the alpha-helical-barrel structures. Whilst this is curiosity driven research, it also has practical consequences for the studies that will follow; that is, alpha-helical barrels made from increasing numbers of helices have channels or pores that increase in a predictable way. Second, we will use rational and empirical design approaches to engineer a range of functions within these cavities, including binding capabilities and enzyme-like activities. Finally, and taking the programme into another ambitious area, we will use the alpha-helical barrels to template other folds that are otherwise difficult to design and engineer, notably beta-barrels that insert into membranes to render ion-channel and sensor functions." Champ scientifique natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsengineering and technologyelectrical engineering, electronic engineering, information engineeringelectronic engineeringsensors Programme(s) FP7-IDEAS-ERC - Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Thème(s) ERC-AG-LS9 - ERC Advanced Grant - Applied life sciences and biotechnology Appel à propositions ERC-2013-ADG Voir d’autres projets de cet appel Régime de financement ERC-AG - ERC Advanced Grant Institution d’accueil UNIVERSITY OF BRISTOL Contribution de l’UE € 2 467 844,00 Adresse BEACON HOUSE QUEENS ROAD BS8 1QU Bristol Royaume-Uni Voir sur la carte Région South West (England) Gloucestershire, Wiltshire and Bristol/Bath area Bristol, City of Type d’activité Higher or Secondary Education Establishments Chercheur principal Derek Neil Woolfson (Prof.) Contact administratif Audrey Michael (Mrs.) Liens Contacter l’organisation Opens in new window Site web Opens in new window Coût total Aucune donnée Bénéficiaires (1) Trier par ordre alphabétique Trier par contribution de l’UE Tout développer Tout réduire UNIVERSITY OF BRISTOL Royaume-Uni Contribution de l’UE € 2 467 844,00 Adresse BEACON HOUSE QUEENS ROAD BS8 1QU Bristol Voir sur la carte Région South West (England) Gloucestershire, Wiltshire and Bristol/Bath area Bristol, City of Type d’activité Higher or Secondary Education Establishments Chercheur principal Derek Neil Woolfson (Prof.) Contact administratif Audrey Michael (Mrs.) Liens Contacter l’organisation Opens in new window Site web Opens in new window Coût total Aucune donnée