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Methods for high-throughput glycoproteomic analysis

Objective

The main aim of the HTP-GlycoMet proposal is to develop technologies, which will enable high-throughput analysis of glycosylation of individual proteins from body fluids and cell membranes and apply them to understand some key processes in immunity and infections. Glycan analysis is extremely demanding from both technological and conceptual aspect and (beside one study performed by partners in this proposal) large-scale studies of glycosylation of individual proteins were not attempted previously. However, we are confident that by successfully combining complementary expertise in the (i) production of specialised monolithic chromatographic tools for high-throughput fractionation of complex biological fluids (BIA-SEP), (ii) purification of proteins from body fluids and membrane proteins (UNI-RI), (iii) high-throughput glycomic analysis by use of chromatography (Genos) and multiplexed capillary gel electrophoresis (MPI) and (iv) expertise in the field of viral immunology (MEDRI) we can achieve significant progress in this direction. All our partners are recognized leaders who already made significant progress beyond the state of the art in their respective fields. Through HTP-GlycoMet project we will organise secondments on all levels (MER, ER, ESR) to capitalize on synergistic effects of this interdisciplinary and transnational collaboration. In addition to the generation of new knowledge and the development of new innovative technologies, we will also achieve significant transfer of knowhow between academic and industrial partners. Our SME partners also expect to develop new lines of products and services through the HTP-GlycoMet programme, but also through future collaboration with HTP-GlycoMet partners beyond the lifetime of this project.

Call for proposal

FP7-PEOPLE-2012-IAPP
See other projects for this call

Coordinator

SVEUCILISTE U RIJECI
Address
Trg Brace Mazuranica 10
51000 Rijeka
Croatia
Activity type
Higher or Secondary Education Establishments
EU contribution
€ 329 349,54
Administrative Contact
Djuro Josic (Prof.)

Participants (4)

BIA SEPARATIONS DOO
Slovenia
EU contribution
€ 273 317,14
Address
Mirce 21
5270 Ajdovscina
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Lidija Urbas (Dr.)
GENOS DOO ZA VJESTACENJE I ANALIZU
Croatia
EU contribution
€ 560 256,86
Address
Vatrogasna 112
31000 Osijek
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Cizmesija Ivana (Mrs.)
MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN EV
Germany
EU contribution
€ 338 055,63
Address
Hofgartenstrasse 8
80539 Munchen
Activity type
Research Organisations
Administrative Contact
Erdmann Rapp (Dr.)
SVEUCILISTE U RIJECI, MEDICINSKI FAKULTET
Croatia
EU contribution
€ 317 098,73
Address
Brace Branchetta 20
51000 Rijeka
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Ani Gerbin (Ms.)