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Optimized esterase biocatalysts for cost-effective industrial production

Objective

OPTIBIOCAT is a 48 months project aimed at developing biocatalysts based on feruloyl esterases (FAEs) and glucuronoyl esterases (GEs) for production of phenolic fatty- and sugar- esters with antioxidant activity for cosmetic industry, expanding the number/type of industrial biotransformations. Selected FAEs and GEs available within the consortium will be improved for their thermo- and solvent- resistance and substrate specificity by site-directed mutagenesis and directed evolution. Novel enzymes will be discovered by mining for new genes from available genomes. An inventory of novel FAEs and GEs will be developed including 50 fungal and 500 bacterial esterases, 25 site-directed and 20 directed evolved mutants. Enzymatic performances will be optimized to enhance the yield (up to the theoretical yield of 100%) and productivity (up to 0.5-1 g/l/h) of reactions giving the main targeted antioxidants: butyl ferulate, p-coumarate, caffeate, sinapate and 5-O-(trans-feruloyl)-arabinofuranose (using FAEs), glucuronate and benzyl glucuronate (using GEs). FAEs and GEs will be also tested for production of other compounds with improved biological activity and properties of hydrophilicity/hydrophobicity for cosmetic applications. Cost-effective methods will be developed for production of the new biocatalysts, in the g/L scale, and for their technical application to produce antioxidants for cosmetic industry, up to 20L. Enzyme immobilization will increase their recyclability up to ten cycles. The ability of the developed catalysts to work in conditions miming the industrial ones with reduced use of solvents and lower temperature than the chemical routes will be demonstrated. The techno-economic viability and environmental friendliness will be assessed considering a full industrial scale scenario. OPTIBIOCAT involves a highly skilled and multidisciplinary partnership of 16 partners from 8 EU countries, and it is a strongly industry driven project through the participation of 8 SMEs and 1 large company.

Field of science

  • /natural sciences/biological sciences/genetics and heredity/genome
  • /natural sciences/biological sciences/biochemistry/biomolecules/proteins/enzymes
  • /social sciences/economics and business/economics/production economics/productivity

Call for proposal

FP7-KBBE-2013-7-single-stage
See other projects for this call

Funding Scheme

CP-TP - Collaborative Project targeted to a special group (such as SMEs)

Coordinator

UNIVERSITA DEGLI STUDI DI NAPOLI FEDERICO II
Address
Corso Umberto I, 40
80138 Napoli
Italy
Activity type
Higher or Secondary Education Establishments
EU contribution
€ 822 319,95
Administrative Contact
Vincenza Faraco (Dr.)

Participants (17)

KORRES S.A. NATURAL PRODUCTS (KORRES ANONIMI ETERIA FYSIKA PROIONTA)
Greece
EU contribution
€ 232 759,60
Address
57Th Km Athens-lamia National Road
32011 Inofyta
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Marianna Ralli (Dr.)
LULEA TEKNISKA UNIVERSITET
Sweden
EU contribution
€ 834 870,40
Address
Universitetsomradet Porson
971 87 Lulea
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Birgitta Karlberg (Mrs.)
Westfälische Wilhelms-Universität Münster
Germany
EU contribution
€ 357 382
Address
Schlossplatz 2
48149 Muenster
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Frank Thuis (Mr.)
SUPREN GMBH
Germany
EU contribution
€ 413 000
Address
Joseph-von-fraunhofer-str. 20
44227 Dortmund
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Axel Gottschalk (Prof.)
TAROS CHEMICALS GMBH & CO KG
Germany
EU contribution
€ 502 880
Address
Emil-figge-str. 76A
44227 Dortmund
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Alexander Piechot (Dr.)
INSTITUT NATIONAL DE RECHERCHE POUR L'AGRICULTURE, L'ALIMENTATION ET L'ENVIRONNEMENT
France
EU contribution
€ 486 348,80
Address
147 Rue De L'universite
75007 Paris Cedex 07
Activity type
Research Organisations
Administrative Contact
Nicolas Combebiac (Mr.)
KONINKLIJKE NEDERLANDSE AKADEMIE VAN WETENSCHAPPEN - KNAW
Netherlands
EU contribution
€ 455 210
Address
Kloveniersburgwal 29 Het Trippenhuis
1011 JV Amsterdam
Activity type
Other
Administrative Contact
Don Van Velzen (Mr.)
CHALMERS TEKNISKA HOEGSKOLA AB
Sweden
EU contribution
€ 446 022,80
Address
-
41296 Goeteborg
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Catrine Andersson (Ms.)
NZYTECH LDA
Portugal
EU contribution
€ 391 200
Address
Estrada Do Paco Do Lumiar Campus Do Lumiar Edificio E - R/c
1649 038 Lisboa
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Carlos Fontes (Prof.)
GENOMIC INVESTMENTS BV

Participation ended

Netherlands
EU contribution
€ 165 120,40
Address
Plesmanlaan 1D
2333 BZ Leiden
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Pascal Berserik (Mr.)
DYADIC NEDERLAND BV

Participation ended

Netherlands
EU contribution
€ 263 849,33
Address
Nieuwe Kanaal 7 S
6709 PA Wageningen
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Sandra Hinz (Dr.)
CLEA TECHNOLOGIES BV

Participation ended

Netherlands
EU contribution
€ 202 275,56
Address
Delftechpark 34
2628 XH Delft
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Roger Sheldon (Prof.)
HELSINGIN YLIOPISTO
Finland
EU contribution
€ 477 680,40
Address
Yliopistonkatu 3
00014 Helsingin Yliopisto
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Pilvi Toppinen (Ms.)
PROTEONIC BV
Netherlands
EU contribution
€ 443 406,40
Address
Niels Bohrweg 11-13
2333 CA Leiden
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Raymond Verhaert (Dr.)
BIOCOM
Germany
EU contribution
€ 231 631,58
Address
Lutzowstrasse 33-36
10785 Berlin
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Boris Mannhardt (Dr.)
GENENCOR INTERNATIONAL BV
Netherlands
EU contribution
€ 125 888,78
Address
Archimedesweg 30
2333 CN Leiden
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Jan Bernard Wevers (Mr.)
GenomeScan B.V.
Netherlands
Address
Plesmanlaan 1D
2333 BZ Leiden
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Zoraide Granchi (Dr.)