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analyse Soluble + Membrane complexes with Improved LILBID Experiments

analyse Soluble + Membrane complexes with Improved LILBID Experiments

Objective

Crucial processes within cells depend on specific non-covalent interactions which mediate the assembly of proteins and other biomolecules. Deriving structural information to understand the function of these complex systems is the primary goal of Structural Biology.
In this application, the recently developed LILBID method (Laser Induced Liquid Bead Ion Desorption) will be optimized for investigation of macromolecular complexes with a mass accuracy two orders of magnitude better than in 1st generation spectrometers.
Controlled disassembly of the multiprotein complexes in the mass spectrometric analysis while keeping the 3D structure intact, will allow for the determination of complex stoichiometry and connectivity of the constituting proteins. Methods for such controlled disassembly will be developed in two separate units of the proposed LILBID spectrometer, in a collision chamber and in a laser dissociation chamber, enabling gas phase dissociation of protein complexes and removal of excess water/buffer molecules. As a third unit, a chamber allowing determination of ion mobility (IM) will be integrated to determine collisional cross sections (CCS). From CCS, unique information regarding the spatial arrangement of proteins in complexes or subcomplexes will then be obtainable from LILBID.
The proposed design of the new spectrometer will offer fundamentally new possibilities for the investigation of non-covalent RNA, soluble and membrane protein complexes, as well as broadening the applicability of non-covalent MS towards supercomplexes.
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Principal Investigator

Nina Morgner (Dr.)

Host institution

JOHANN WOLFGANG GOETHE-UNIVERSITATFRANKFURT AM MAIN

Address

Theodor W Adorno Platz 1
60323 Frankfurt Am Main

Germany

Activity type

Higher or Secondary Education Establishments

EU Contribution

€ 1 264 477

Principal Investigator

Nina Morgner (Dr.)

Administrative Contact

Kristina Wege (Ms.)

Beneficiaries (1)

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JOHANN WOLFGANG GOETHE-UNIVERSITATFRANKFURT AM MAIN

Germany

EU Contribution

€ 1 264 477

Project information

Grant agreement ID: 337567

Status

Closed project

  • Start date

    1 February 2014

  • End date

    31 January 2019

Funded under:

FP7-IDEAS-ERC

  • Overall budget:

    € 1 264 477

  • EU contribution

    € 1 264 477

Hosted by:

JOHANN WOLFGANG GOETHE-UNIVERSITATFRANKFURT AM MAIN

Germany