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analyse Soluble + Membrane complexes with Improved LILBID Experiments

Ziel

Crucial processes within cells depend on specific non-covalent interactions which mediate the assembly of proteins and other biomolecules. Deriving structural information to understand the function of these complex systems is the primary goal of Structural Biology.
In this application, the recently developed LILBID method (Laser Induced Liquid Bead Ion Desorption) will be optimized for investigation of macromolecular complexes with a mass accuracy two orders of magnitude better than in 1st generation spectrometers.
Controlled disassembly of the multiprotein complexes in the mass spectrometric analysis while keeping the 3D structure intact, will allow for the determination of complex stoichiometry and connectivity of the constituting proteins. Methods for such controlled disassembly will be developed in two separate units of the proposed LILBID spectrometer, in a collision chamber and in a laser dissociation chamber, enabling gas phase dissociation of protein complexes and removal of excess water/buffer molecules. As a third unit, a chamber allowing determination of ion mobility (IM) will be integrated to determine collisional cross sections (CCS). From CCS, unique information regarding the spatial arrangement of proteins in complexes or subcomplexes will then be obtainable from LILBID.
The proposed design of the new spectrometer will offer fundamentally new possibilities for the investigation of non-covalent RNA, soluble and membrane protein complexes, as well as broadening the applicability of non-covalent MS towards supercomplexes.

Aufforderung zur Vorschlagseinreichung

ERC-2013-StG
Andere Projekte für diesen Aufruf anzeigen

Gastgebende Einrichtung

JOHANN WOLFGANG GOETHE-UNIVERSITAET FRANKFURT AM MAIN
Adresse
Theodor W Adorno Platz 1
60323 Frankfurt Am Main
Deutschland
Aktivitätstyp
Higher or Secondary Education Establishments
EU-Beitrag
€ 1 264 477
Hauptforscher
Nina Morgner (Dr.)
Kontakt Verwaltung
Kristina Wege (Ms.)

Begünstigte (1)

JOHANN WOLFGANG GOETHE-UNIVERSITAET FRANKFURT AM MAIN
Deutschland
EU-Beitrag
€ 1 264 477
Adresse
Theodor W Adorno Platz 1
60323 Frankfurt Am Main
Aktivitätstyp
Higher or Secondary Education Establishments
Hauptforscher
Nina Morgner (Dr.)
Kontakt Verwaltung
Kristina Wege (Ms.)