GENETIC EFFECTS OF ENVIRONMENTAL CHEMICALS

Ziel

1) STUDY OF MOLECULAR INTERACTIONS OF ENVIRONMENTAL MUTAGENS AND CARCINOGENS AT DNA LEVEL. 2) TO RELATE BIOLOGICAL CONSEQUENCES OF CHEMICAL MUTAGENS TO THE EXTENT AND SITE OF REACTION BETWEEN THE MUTAGENS AND DNA.
Monoclonal antibodies (MAB) specific for several alkylated deoxyribonucleic acid (DNA) components were characterised for sensitivity and cross reactivity. These MABs were found to be highly specific and suitable for detecting alkylated DNA components in human urine samples, even in the presence of a large excess of other purines. These MABs will enable the development of a rapid monitoring system for the detection and quantitation of 3-alkyladenine in DNA, ribonucleic acid (RNA) and body fluids of individuals previously exposed to alkylating agents.
1) USING (ALKYL-DEOXYNUCLEOSIS)-SPECIFIC MAB IN COMBINATION WITH APPROPRIATE IMMUNOANALYTICAL TECHNIQUES, THE MOLECULAR MECHANISMS OF THE INTERACTION OF ALKYLATING CARCINOGENS WITH GENOMIC DNA OF TARGET CELLS, AS WELL AS THE DIFFERENTIAL CAPACITY OF MAMMALIAN (HUMAN) CELLS TO ENZYMATICALLY DETECT AND REMOVE SPECIFIC ALKYLATION PRODUCTS IN/FROM CELLULAR DNA WILL BE ANALYZED.

2) THE SPECTRUM OF HIGH AFFINITY MAB'S WILL BE EXPANDED TO INCLUDE MAB'S SPECIFIC FOR SOME OF THE REACTION PRODUCTS OF ALKYLATING AGENTS WITHS DNA, IN PARTICULAR THOSE USED IN THE MOLECULAR DOSIMETRY PROGRAMME.

Wissenschaftliches Gebiet

Programm/Programme

FP1-ENVPROT 4C - Multiannual research and development programmes (EEC) in the field of the environment - Protection of the environment -, 1986-1990

Thema/Themen

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Aufforderung zur Vorschlagseinreichung

Data not available

Finanzierungsplan

CSC - Cost-sharing contracts

Koordinator

Universität-Gesamthochschule Essen

EU-Beitrag

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Adresse

Hufelandstraße 55
45147 Essen
Deutschland

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