Objective - BEST UNDERSTANDING OF THE ANTIGENIC POLYMORPHISM OF AFRICAN SWINE FEVER. - EVENTUALLY, PRODUCTION OF A VACCINE AGAINST THIS DISEASE. African swine fever (ASF) is an infectious disease of domestic pigs and acari of the genus Ornithodoros. In the pig, the major target cell is the macrophage and research has been carried out to see whether or not the porcine macrophage has a specific receptor for the virus and, if so, to identify the virus attachment protein, to map the gene, to sequence and express it in sufficient amounts to carry out protection experiments in pigs. Porcine macrophages, but not macrophages from animal species resistant to the virus, have been found to have a specific receptor for ASF virus. The attachment of the virus to the macrophage occurred through an external protein (p12) in the virion, and this interaction was necessary to induce infection. The gene coding for protein p12 has been mapped and sequenced and work is in progress to express the gene to obtain enough protein p12 to carry out protection experiments.THE AIM OF THIS PROJECT IS TO ISOLATE A PROTEIN CAPABLE TO INDUCE A PROTECTIVE RESPONSE AGAINST ASF VIRUS. THE PROJECT CONSIST IN FOUR STEPS : - IDENTIFICATION OF VIRAL PROTEINS EXPOSED IN VIRIONS AND VIRUS INFECTED CELLS. - ISOLATION OF MONOCLONAL AND MONOSPECIFIC ANTIBODIES. - GENE MAPPING. - NEUTRALIZING AND PROTECTING ANTIBODIES. Fields of science natural sciencesbiological sciencesmicrobiologyvirologymedical and health scienceshealth sciencesinfectious diseasesnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsmedical and health sciencesbasic medicineimmunologymedical and health sciencesbasic medicinepharmacology and pharmacypharmaceutical drugsvaccines Programme(s) FP1-BAP - Multiannual research action programme (EEC) in the field of biotechnology (BAP), 1985-1989 Topic(s) Data not available Call for proposal Data not available Funding Scheme CSC - Cost-sharing contracts Coordinator CONSEJO SUP.INV.CIENTIFICAS / UNIVERSITAD AUTONOMA DE MADRID EU contribution No data Address CANTO BLANCO MADRID 34 Spain See on map Total cost No data
