Objectif A NEW THERMOPHILIC FUNGUS, CL240, WAS SELECTED IN A SCREENING PROGRAM FOR ITS ABILITY TO PRODUCE A VERY BROAD SPECTRUM OF POLYSACCHAROLYTIC ACTIVITIES: CELLULASES, VARIOUS HEMICELLULASES, BETA GLUCANASES, AMYLOGLUCOSIDASE... ALL THESE ENZYMES ARE STABLE AT 70 CELSIUS DEGREES OR MORE. THE SPECIFIC ACTIVITY OF THE CELLULOLYTIC COMPLEX ON CRISTALLINE CELLULOSE, AND THE PRODUCTIVITY OF WILD TYPE STRAINS COMPARE FAVOURABLY WITH THOSE OF TRICHODERMA REESEI. THE AIM OF THIS RESEARCH PROPOSAL IS THE SCREENING OF MUTANTS OF THIS STRAIN WHOSE SPECIFIC ACTIVITY OF EXTRACELLULAR PROTEINS TOWARDS CRISTALLINE CELLULOSE WILL BE HIGHER THAN THAT OF CELLULASE FROM TRICHODERMA REESEI. CELLULOLYTIC ENZYMES WILL BE PURIFIED BY PREPARATIVE CHROMATOGRAPHY AND THE BEST COMBINATION OF EACH COMPONENT TO GIVE AN INCREASE OF THE SPECIFIC ACTIVITY WILL BE DETERMINED. THE SEARCH OF MUTANTS WILL THEN BE ORIENTED TOWARDS A SPECIFIC INCREASE OF COMPONENTS SUCH AS EXOGLUCANASES WHOSE CONCENTRATION IS LIMITING IN THE CELLULOLYTIC COMPLEX. SELECTION OF MUTANTS WILL BE CARRIED OUT BY CONVENTIONNAL GENETIC PROCEDURE USING ADAPTED SCREENS. THE EFFICIENCY OF ENZYME PREPARATIONS FROM DIFFERENT MUTANTS IN CONVERTING STEAM EXPLODED CORN STOVERS TO ALCOHOLS FERMENTABLE SUGARS WILL BE ASSESSED. Champ scientifique natural sciencesbiological sciencesmicrobiologymycologysocial scienceseconomics and businesseconomicsproduction economicsproductivitynatural scienceschemical sciencesorganic chemistryalcoholsnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsenzymes Programme(s) FP1-ENNONUC 3C - Research and development programme (EEC) in the field of Non-Nuclear Energy, 1985-1988 Thème(s) Data not available Appel à propositions Data not available Régime de financement CSC - Cost-sharing contracts Coordinateur Centre National de la Recherche Scientifique (CNRS) Contribution de l’UE Aucune donnée Adresse 118 route de Narbonne 31062 Toulouse France Voir sur la carte Coût total Aucune donnée