Skip to main content
European Commission logo print header

BIOCHEMICAL AND GENETICAL ANALYSIS OF CELLULOLYTIC ACTIVITIES OF A THERMOPHILIC FUNGUS BELONGING TO THE TALAROMYCES GENUS.

Objectif

A NEW THERMOPHILIC FUNGUS, CL240, WAS SELECTED IN A SCREENING PROGRAM FOR ITS ABILITY TO PRODUCE A VERY BROAD SPECTRUM OF POLYSACCHAROLYTIC ACTIVITIES: CELLULASES, VARIOUS HEMICELLULASES, BETA GLUCANASES, AMYLOGLUCOSIDASE... ALL THESE ENZYMES ARE STABLE AT 70 CELSIUS DEGREES OR MORE. THE SPECIFIC ACTIVITY OF THE CELLULOLYTIC COMPLEX ON CRISTALLINE CELLULOSE, AND THE PRODUCTIVITY OF WILD TYPE STRAINS COMPARE FAVOURABLY WITH THOSE OF TRICHODERMA REESEI.

THE AIM OF THIS RESEARCH PROPOSAL IS THE SCREENING OF MUTANTS OF THIS STRAIN WHOSE SPECIFIC ACTIVITY OF EXTRACELLULAR PROTEINS TOWARDS CRISTALLINE CELLULOSE WILL BE HIGHER THAN THAT OF CELLULASE FROM TRICHODERMA REESEI. CELLULOLYTIC ENZYMES WILL BE PURIFIED BY PREPARATIVE CHROMATOGRAPHY AND THE BEST COMBINATION OF EACH COMPONENT TO GIVE AN INCREASE OF THE SPECIFIC ACTIVITY WILL BE DETERMINED. THE SEARCH OF MUTANTS WILL THEN BE ORIENTED TOWARDS A SPECIFIC INCREASE OF COMPONENTS SUCH AS EXOGLUCANASES WHOSE CONCENTRATION IS LIMITING IN THE CELLULOLYTIC COMPLEX. SELECTION OF MUTANTS WILL BE CARRIED OUT BY CONVENTIONNAL GENETIC PROCEDURE USING ADAPTED SCREENS.
THE EFFICIENCY OF ENZYME PREPARATIONS FROM DIFFERENT MUTANTS IN CONVERTING STEAM EXPLODED CORN STOVERS TO ALCOHOLS FERMENTABLE SUGARS WILL BE ASSESSED.

Thème(s)

Data not available

Appel à propositions

Data not available

Régime de financement

CSC - Cost-sharing contracts

Coordinateur

Centre National de la Recherche Scientifique (CNRS)
Contribution de l’UE
Aucune donnée
Adresse
118 route de Narbonne
31062 Toulouse
France

Voir sur la carte

Coût total
Aucune donnée