The project aims at an elucidation of the molecular structure and molecular dynamics of the yeast plasma membrane H+-ATPase. This study represents a key stage to understand the general mechanism of active transport. The main objectives of this research will be the
understanding of how the phosphate transfer reaction is coupled to proton binding and transpor and how is the plasma membrane ATPase regulated. Crystals of the H+-ATPase in different states and the following X-ray structure analysis should shine additional light on the mechanism of this enzyme.