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Selective breeding and genetic management of fish through genome marking and inbred clones.

Ziel

The general aim of this project is to develop new approaches to genetic management and selective breeding in fish by means of recent advances in molecular genetics and chromosome manipulations. Rainbow trout and Atlantic salmon will be chosen as species models.

The first step of this project will consist in cloning a large number of DNA probes which will allow to describe the genetic variability at the DNA level. These methods will provide, together with protein polymorphism studies, basic informations which could be immediately applied to:

description of gene diversity of domesticated and natural populations and, if necessary, introduction of new genetic material in fish-farm stocks from the wild,
evaluation of impact of fish-farm escapes on wild populations,
conservation of genetic resources of species.

The mid-step goal will be the construction of a preliminary linkage map of the polymorphic enzyme loci and DNA sites detected with the salmonid sequences cloned in step 1 and the long-term goal will be the use of this linkage map for the detection of major genes coding for quantitative traits in rainbow trout and Atlantic salmon. The biological material used for these two goals will be full-sib families and homozygous rainbow trout lines which will be obtained by chromosome manipulations.

The project can be divided into three major interconnected phases:

DNA probe cloning,
description of RFLPs (restriction fragment length polymorphism) and enzyme variation,
construction of a preliminary linkage map and analysis of linkage between RFLPs and QTLs (quantitative trait loci).

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Finanzierungsplan

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Koordinator

Institut National de la Recherche Agronomique (INRA)
EU-Beitrag
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Adresse
Domaine de Vilvert
78352 Jouy-en-Josas
Frankreich

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