Cel The cellular environment is extremely complex and contains thousands of different bio-molecules. To determine how these bio-molecules assemble into a highly organized molecular system has remained a major challenge. Here, we aim to unravel how dozens of proteins assemble into a large molecular machinery that degrades mRNA in an efficient and regulated manner.On an atomic level, we will determine the static structures of the enzymes involved in the mRNA degradation pathways. In parallel, we will develop and exploit novel methods in NMR spectroscopy to correlate molecular motions with catalytic activity. Especially for the 450 kDa eukaryotic exosome complex, these studies will significantly push the limits of what is currently achievable. On a molecular level, we will reconstitute the mRNA degradation machinery from purified components in a stepwise manner. In this challenging approach, we will use NMR spectroscopy to follow how the enzymes engage in a network of interactions that regulate the mRNA degradation process. These studies will also provide us with the unique possibility to reproduce complex cellular behavior in a well defined and easy to manipulate in vitro system. On a microscopic level the mRNA degradation machinery assembles into cytoplasmic processing bodies. The role of these conserved cellular foci is a matter of debate and we aim to determine both the atomic details that result in this self-organization as well as the catalytic advantages that result from this clustering.Our study will provide a very detailed and accurate description of how essential and central molecular processes in mRNA degradation are regulated and modulated. The level of detail that we aim to achieve is currently not available for any cellular pathway of such complexity. In that regard, our projects also provide knowledge and methodology required to study additional and complex cellular functions. Dziedzina nauki natural sciencesphysical sciencesopticsspectroscopyabsorption spectroscopynatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsenzymes Program(-y) FP7-IDEAS-ERC - Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Temat(-y) ERC-CG-2013-LS1 - ERC Consolidator Grant - Molecular and Structural Biology and Biochemistry Zaproszenie do składania wniosków ERC-2013-CoG Zobacz inne projekty w ramach tego zaproszenia System finansowania ERC-CG - ERC Consolidator Grants Instytucja przyjmująca UNIVERSITAET REGENSBURG Wkład UE € 1 089 318,89 Adres UNIVERSITATSSTRASSE 31 93053 Regensburg Niemcy Zobacz na mapie Region Bayern Oberpfalz Regensburg, Kreisfreie Stadt Rodzaj działalności Higher or Secondary Education Establishments Kontakt administracyjny Matthias Köhler (Dr.) Kierownik naukowy Remco Sprangers (Dr.) Linki Kontakt z organizacją Opens in new window Strona internetowa Opens in new window Koszt całkowity Brak danych Beneficjenci (2) Sortuj alfabetycznie Sortuj według wkładu UE Rozwiń wszystko Zwiń wszystko UNIVERSITAET REGENSBURG Niemcy Wkład UE € 1 089 318,89 Adres UNIVERSITATSSTRASSE 31 93053 Regensburg Zobacz na mapie Region Bayern Oberpfalz Regensburg, Kreisfreie Stadt Rodzaj działalności Higher or Secondary Education Establishments Kontakt administracyjny Matthias Köhler (Dr.) Kierownik naukowy Remco Sprangers (Dr.) Linki Kontakt z organizacją Opens in new window Strona internetowa Opens in new window Koszt całkowity Brak danych MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN EV Zakończenie uczestnictwa Niemcy Wkład UE € 877 481,11 Adres HOFGARTENSTRASSE 8 80539 Munchen Zobacz na mapie Region Bayern Oberbayern München, Kreisfreie Stadt Rodzaj działalności Research Organisations Kontakt administracyjny Antje Lemper-Rupp (Mrs.) Linki Kontakt z organizacją Opens in new window Strona internetowa Opens in new window Koszt całkowity Brak danych
