CORDIS
EU research results

CORDIS

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Dynamic signalling networks in Diabetic Nephropathy (DN)<br/>– New avenues to a personalized therapy.-

Dynamic signalling networks in Diabetic Nephropathy (DN)
– New avenues to a personalized therapy.-

Objective

Dynamic signalling networks in Diabetic Nephropathy (DN) – New avenues to a personalized therapy.-
We have developed an exquisite experimental platform that facilitates the systematic unravelling of the signalling
networks leading to (1) the initiation, (2) the progression and (3) the potential regeneration of podocytes in
DN, paving the way to novel therapeutic strategies:
(1) DN initiation: Identification of signalling cascades leading to microalbuminuria: Molecular
By combining transgenic Drosophila lines carrying secreted fluorescent proteins to monitor the barrier function
in vivo with a genome-wide siRNA screen we will establish a unique system to directly identify gene
networks contributing to microalbuminuria.
(2a) DN progression: Molecular fingerprinting of podocyte degeneration: Based on a transgenic
fluorescent mouse model, we have pioneered a highly efficient podocyte purification method from type1 and
type 2 diabetic mice allowing us to develop a precise molecular genetic, quantitative proteomic and micro
RNA fingerprint from freshly isolated podocytes from diabetic and non-diabetic mice.
(2b) DN progression: We established a proteomic approach to measure site-specific phosphorylation dynamics in
primary podocyte cultures originating from transgenic mice that are TORC1 deficient, TORC2 deficient or
TORC1 hyperactive (TSC1 KO) solely in the podocytes.
(3) Potential role of podocyte regeneration in DN: Finally, to target mechanisms that could potentially
reverse the disease process (by repopulating lost podocytes), we invented a strategy to quantitatively monitor
podocyte turnover from different stem cell niches allowing us to precisely assess and potentially
manipulating the capacity of podocyte regeneration in DN.
Leaflet | Map data © OpenStreetMap contributors, Credit: EC-GISCO, © EuroGeographics for the administrative boundaries

Principal Investigator

Tobias Georg Bruno Maria Huber (Prof.)

Host institution

UNIVERSITAETSKLINIKUM HAMBURG-EPPENDORF

Address

Martinistrasse 52
20251 Hamburg

Germany

Activity type

Higher or Secondary Education Establishments

EU Contribution

€ 739 672,74

Principal Investigator

Tobias Georg Bruno Maria Huber (Prof.)

Administrative Contact

Tobias Huber (Prof.)

Beneficiaries (2)

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UNIVERSITAETSKLINIKUM HAMBURG-EPPENDORF

Germany

EU Contribution

€ 739 672,74

UNIVERSITAETSKLINIKUM FREIBURG

Germany

EU Contribution

€ 1 260 247,26

Project information

Grant agreement ID: 616891

Status

Closed project

  • Start date

    1 June 2014

  • End date

    31 May 2019

Funded under:

FP7-IDEAS-ERC

  • Overall budget:

    € 1 999 920

  • EU contribution

    € 1 999 920

Hosted by:

UNIVERSITAETSKLINIKUM HAMBURG-EPPENDORF

Germany