Obiettivo Unfolded protein response (UPR) is activated by multiple types of cellular stress, and can promote either cell survival or apoptosis. The balance between these opposing outcomes is delicately regulated, and when lost, contributes to diverse diseases. UPR enables cells to halt general translation, while inducing translation and transcription of specific mRNAs that escape repression. Even though the general machinery controlling translation is well understood, several fundamental open questions remain: 1) how are mRNAs selected for translation during UPR, 2) what role does mRNA structure and sequence play in this selection, 3) what role does UPR pathway play in the highly differentiated cells, such as neurons? My lab employs an integrative approach to understand how RNA-binding proteins (RBPs) control specific mRNAs. We recently developed hiCLIP, a method that globally quantifies interactions between RBPs and double-stranded RNA in live cells. Our preliminary findings demonstrate that a double-stranded RBP binds to structured motifs in mRNAs to control stress-induced translation. I propose to determine how combinatorial recognition of RNA sequence and structure by RBPs controls mRNA localisation, stability and translation during UPR. In addition, we will assess the role of UPR pathway in neuronal differentiation. Taken together, this study aims to elucidate how cells select specific mRNAs for translation, and thereby survive during stress or respond to signals that control differentiation. Campo scientifico natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsnatural sciencesbiological sciencesgeneticsRNA Programma(i) FP7-IDEAS-ERC - Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Argomento(i) ERC-CG-2013-LS1 - ERC Consolidator Grant - Molecular and Structural Biology and Biochemistry Invito a presentare proposte ERC-2013-CoG Vedi altri progetti per questo bando Meccanismo di finanziamento ERC-CG - ERC Consolidator Grants Istituzione ospitante UNIVERSITY COLLEGE LONDON Contributo UE € 1 999 435,00 Indirizzo GOWER STREET WC1E 6BT LONDON Mostra sulla mappa Tipo di attività Higher or Secondary Education Establishments Contatto amministrativo Giles Machell (Mr.) Ricercatore principale Jernej Ule (Prof.) Collegamenti Contatta l’organizzazione Opens in new window Sito web Opens in new window Costo totale Nessun dato Beneficiari (1) Classifica in ordine alfabetico Classifica per Contributo UE Espandi tutto Riduci tutto UNIVERSITY COLLEGE LONDON Contributo UE € 1 999 435,00 Indirizzo GOWER STREET WC1E 6BT LONDON Mostra sulla mappa Tipo di attività Higher or Secondary Education Establishments Contatto amministrativo Giles Machell (Mr.) Ricercatore principale Jernej Ule (Prof.) Collegamenti Contatta l’organizzazione Opens in new window Sito web Opens in new window Costo totale Nessun dato