CORDIS
EU research results

CORDIS

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Expression and function of globin X in nucleated red blood cells of fish

Project information

Grant agreement ID: 623338

Status

Closed project

  • Start date

    1 August 2014

  • End date

    31 July 2016

Funded under:

FP7-PEOPLE

  • Overall budget:

    € 276 865,20

  • EU contribution

    € 276 865,20

Coordinated by:

TURUN YLIOPISTO

Finland

Objective

The aim of the project is to understand the function of globin X (GbX) in the activation of the sodium/proton exchange across the erythrocyte membrane of fish. This exchange is oxygen dependent and activated by adrenergic stimulation; however the exact mechanism is unknown. It has been suggested that membrane binding of haemoglobin would be involved but such haemoglobin has not been found yet.
Recently we found that GbX mRNA is expressed in red blood cells of fish. GbX is a membrane-bound vertebrate globin that is unique within the vertebrate (haemo-) globin superfamily and its cellular functions are not well understood because practically all available data on GbX are based on mRNA abundance.
In a multidisciplinary approach we will combine the molecular and functional findings on GbX and oxygen-dependent membrane transport in a completely new fashion with an environmental focus. The study includes in vitro approaches in red blood cell culture and in vivo experiments in different fish species to unravel the cellular functions of GbX. The central research question is if GbX regulates the oxygen affinity of haemoglobin by affecting the ion transport in nucleated erythrocytes of fish.
The research proposed in this proposal has the potential to make a valuable contribution to the field of red blood cell research by applying state-of-the-art techniques which have not been applied to nucleated erythrocytes before. The underlying aims are to explain the molecular basis of oxygen transport and to unravel adaptation mechanisms of fish species to environmental challenges. The project thereby touches several of the biggest issues today since global warming, oxygen limitation and pollution highly impact aquatic ecosystems worldwide. In particular marginal Seas such as the Baltic Sea, the second largest brackish water area in the world, are affected. The host of the project will be Prof Mikko Nikinmaa at the University of Turku, Finland.
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Coordinator

TURUN YLIOPISTO

Address

Yliopistonmaki
20014 Turku

Finland

Activity type

Higher or Secondary Education Establishments

EU Contribution

€ 276 865,20

Administrative Contact

Mikko Nikinmaa (Prof.)

Project information

Grant agreement ID: 623338

Status

Closed project

  • Start date

    1 August 2014

  • End date

    31 July 2016

Funded under:

FP7-PEOPLE

  • Overall budget:

    € 276 865,20

  • EU contribution

    € 276 865,20

Coordinated by:

TURUN YLIOPISTO

Finland