Objetivo N-glycosylation consists in the addition of a glycan precursor onto a nascent protein in the endoplasmic reticulum. This glycan is then trimmed and matured in the Golgi by addition of various sugars motifs, leading to a large heterogeneity of glycans structures, from site to site or on the same glycosylation site. Until now the underlying causes of this micro-heterogeneity have not clearly been identified and the influence of the sugar-nucleotides pool and the glycosyltransferases present in the cell was proposed.By using high-performance technologies such as mass spectrometry (LC-MS-MS), NMR and crystallography, and by using classic biochemical and molecular biology approaches to modify the glycan and/ or the structure of a set of model proteins, we propose here to prove that heterogeneity of glycan structures is also highly influenced by the three-dimensional protein structure and the interaction between the protein and the glycan.Since glycosylation can be involved in protein: folding, maturation, secretion and/or functions, the appreciation of heterogeneity mechanism is crucial to better apprehend the role of the glycans at a cellular level. In addition of paving the way to a better comprehension of the glycan structures role , this challenging project will impact European pharmaceutical company producing recombinant glycoproteins or glycopeptides as drugs, by giving them answers on how to reduce the glycan heterogeneity without multiplying high-cost purification steps.Keywords: glycosylation; biochemistry; recombinant protein Ámbito científico natural sciencesearth and related environmental sciencesgeologymineralogycrystallographynatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsnatural sciencesbiological sciencesbiochemistrybiomoleculescarbohydratesnatural scienceschemical sciencesanalytical chemistrymass spectrometrynatural sciencesbiological sciencesmolecular biology Programa(s) FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Tema(s) FP7-PEOPLE-2013-IEF - Marie-Curie Action: "Intra-European fellowships for career development" Convocatoria de propuestas FP7-PEOPLE-2013-IEF Consulte otros proyectos de esta convocatoria Régimen de financiación MC-IEF - Intra-European Fellowships (IEF) Coordinador EIDGENOESSISCHE TECHNISCHE HOCHSCHULE ZUERICH Aportación de la UE € 199 317,60 Dirección Raemistrasse 101 8092 Zuerich Suiza Ver en el mapa Región Schweiz/Suisse/Svizzera Zürich Zürich Tipo de actividad Higher or Secondary Education Establishments Contacto administrativo Markus Aebi (Prof.) Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Coste total Sin datos
