Obiettivo Polyacrylamide gel electrophoresis (PAGE) is the most widespread tool to separate and detect macromolecules based on their molecular weight. New generation PAGE assays miniaturize the technique to obtain high throughput performance and reduce sample volume, reagents and cost. Here, we present a novel technology that will improve the range, the resolution and the accuracy that these new generation PAGE assays can resolve. On traditional PAGE assays, polyacrylamide gels with an acrylamide gradient are used to significantly improve the separation of macromolecules. However, on new generation PAGE assays the technology to fabricate those gradients at the millimetre scale with micron resolution is missing. The technology presented in this project, developed as a part of the ERC project GenesForceMotion, allows the fabrication of millimetre sized polyacrylamide gels with a well-defined and precise acrylamide microgradient. Moreover, the acrylamide density profile can be precisely controlled along the gel to improve the separation of macromolecules with similar molecular weights. This technology generates a market opportunity to improve the performance of the gel electrophoresis technique widely extended in worldwide life science laboratories. The expected outcome of the project is to test the benefits of our microgradient gel technology to improve the new generation miniaturized PAGE assays and make this technology available to the market Campo scientifico natural scienceschemical scienceselectrochemistryelectrophoresis Programma(i) FP7-IDEAS-ERC - Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Argomento(i) ERC-OA-2013-PoC - European Research Council ERC Proof of Concept Invito a presentare proposte ERC-2013-PoC Vedi altri progetti per questo bando Meccanismo di finanziamento CSA-SA(POC) - Supporting action (Proof of Concept) Istituzione ospitante FUNDACIO INSTITUT DE BIOENGINYERIA DE CATALUNYA Contributo UE € 148 962,65 Indirizzo CARRER BALDIRI REIXAC PLANTA 2A 10-12 08028 Barcelona Spagna Mostra sulla mappa Regione Este Cataluña Barcelona Tipo di attività Research Organisations Contatto amministrativo Josep Samitier (Prof.) Collegamenti Contatta l’organizzazione Opens in new window Sito web Opens in new window Costo totale Nessun dato Beneficiari (1) Classifica in ordine alfabetico Classifica per Contributo UE Espandi tutto Riduci tutto FUNDACIO INSTITUT DE BIOENGINYERIA DE CATALUNYA Spagna Contributo UE € 148 962,65 Indirizzo CARRER BALDIRI REIXAC PLANTA 2A 10-12 08028 Barcelona Mostra sulla mappa Regione Este Cataluña Barcelona Tipo di attività Research Organisations Contatto amministrativo Josep Samitier (Prof.) Collegamenti Contatta l’organizzazione Opens in new window Sito web Opens in new window Costo totale Nessun dato
