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Exploiting native endowments by re-factoring, re-programming and implementing novel control loops in Pseudomonas putida for bespoke biocatalysis

Objective

We aim to engineer the lifestyle of Pseudomonas putida to generate a tailored, re-factored chassis with highly attractive new-to-nature properties, thereby opening the door to the production of thus far non-accessible compounds. This industrially driven project capitalises on the outstanding metabolic endowment and stress tolerance capabilities of this versatile bacterium for the production of specialty and bulk chemicals. Specifically, we will build streamlined P. putida strains with improved ATP availability utilizing this power on demand, decoupled from growth. The well-characterized, streamlined and re-factored strain platform will offer easy-to-use plug-in opportunities for novel, DNA-encoded functions under the control of orthogonal regulatory systems. To this end, we will deploy a concerted approach of genome refactoring, model-driven circuit design, implementation of ATP control loops, structured modelling and metabolic engineering. By drawing on a starkly improved, growth-uncoupled ATP-biosynthetic machinery, empowered P. putida strains will be able to produce a) n-butanol and isobutanol and their challenging gaseous derivatives 1-butene (BE) and (iso-)butadiene (BDE) using a novel, new-to-nature route starting from glucose, as well as b) new active ingredients for crop protection, such as tabtoxin, a high-value, ß-lactam-based secondary metabolite with a huge potential as a new herbicide. The game-changing innovations brought in – in particular the uncoupling of ATP-synthesis and production from growth - will provide strong versatility, enhanced efficiency and efficacy to the production processes, thereby overcoming current bottlenecks, matching market needs and fostering high-level research growth and development.

Call for proposal

H2020-BIOTEC-2014-2015

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Sub call

H2020-LEIT-BIO-2014-1

Coordinator

WAGENINGEN UNIVERSITY
Net EU contribution
€ 1 357 753,64
Address
Droevendaalsesteeg 4
6708 PB Wageningen
Netherlands

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Region
Oost-Nederland Gelderland Veluwe
Activity type
Higher or Secondary Education Establishments
Non-EU contribution
€ 0,00

Participants (9)

UNIVERSITY OF STUTTGART
Germany
Net EU contribution
€ 1 348 266,25
Address
Keplerstrasse 7
70174 Stuttgart

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Region
Baden-Württemberg Stuttgart Stuttgart, Stadtkreis
Activity type
Higher or Secondary Education Establishments
Non-EU contribution
€ 0,00
AGENCIA ESTATAL CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS
Spain
Net EU contribution
€ 721 875,00
Address
Calle Serrano 117
28006 Madrid

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Region
Comunidad de Madrid Comunidad de Madrid Madrid
Activity type
Research Organisations
Non-EU contribution
€ 0,00
EIDGENOESSISCHE TECHNISCHE HOCHSCHULE ZUERICH
Switzerland
Net EU contribution
€ 0,00
Address
Raemistrasse 101
8092 Zuerich

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Region
Schweiz/Suisse/Svizzera Zürich Zürich
Activity type
Higher or Secondary Education Establishments
Non-EU contribution
€ 818 848,00
UNIVERSIDADE NOVA DE LISBOA
Portugal
Net EU contribution
€ 621 250,00
Address
Campus De Campolide
1099 085 Lisboa

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Region
Continente Área Metropolitana de Lisboa Área Metropolitana de Lisboa
Activity type
Higher or Secondary Education Establishments
Non-EU contribution
€ 0,00
INGENZA LIMITED
United Kingdom
Net EU contribution
€ 614 500,00
Address
Roslin Innovation Centre, Charnock Bradley Buildin Easter Bush Campus, Bush Farm Road,
EH25 9RG Roslin

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SME

The organization defined itself as SME (small and medium-sized enterprise) at the time the Grant Agreement was signed.

Yes
Region
Eastern Scotland East Lothian and Midlothian
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Non-EU contribution
€ 0,00
LIFEGLIMMER GMBH
Germany
Net EU contribution
€ 581 750,00
Address
Markelstrasse 38
12163 Berlin

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SME

The organization defined itself as SME (small and medium-sized enterprise) at the time the Grant Agreement was signed.

Yes
Region
Berlin Berlin Berlin
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Non-EU contribution
€ 0,00
ABENGOA RESEARCH SL

Participation ended

Spain
Net EU contribution
€ 128 217,61
Address
Calle Energia Solar 1 - Campus Palmas Altas Sect Sunp Gu I Parcela Ze 3
41014 Sevilla

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Region
Andalucía Sevilla
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Non-EU contribution
€ 0,00
BASF SE
Germany
Net EU contribution
€ 592 212,50
Address
Carl Bosch Strasse 38
67063 Ludwigshafen Am Rhein

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Region
Baden-Württemberg Stuttgart Stuttgart, Stadtkreis
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Non-EU contribution
€ 0,00
LUCITE INTERNATIONAL UK LIMITED
United Kingdom
Net EU contribution
€ 55 000,00
Address
Cassel Works New Road
TS23 1LE Billingham

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Region
North East (England) Tees Valley and Durham Hartlepool and Stockton-on-Tees
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Non-EU contribution
€ 0,00