Periodic Reporting for period 3 - MITOsmORFs (Identification and analysis of novel mitochondrial proteins encoded by small open reading frames)
Reporting period: 2018-09-01 to 2020-02-29
Mitochondria possess a remarkably high content of small proteins compared to other cellular compartments. About one third of the cellular proteins ≤15 kDa characterized to date are located in mitochondria. Functional examples of such small proteins cover the whole mitochondrial biology like cristae morphology, Fe-S cluster formation, metabolite transport, protein biogenesis and respiration. However, the intracellular localization and function of many small proteins is unknown. At the beginning of the project these small proteins constitute one third of the uncharacterized open reading frames in the model organism baker’s yeast. We predict that more than 10% of the mitochondrial proteome deserves to be discovered.
To understand mitochondrial functions, it is absolutely crucial to identify the mitochondrial protein composition. MITOsmORFs aims to identify over 100 novel mitochondrial proteins. In addition, MITOsmORFs will determine the submitochondrial localization and the functional role of mitochondrial proteins. This includes interaction mapping and characterization of mitochondrial activities in vivo and in organello to explore the unknown biology mitochondrial proteins.