Periodic Reporting for period 1 - MRTGS (Mechanical regulation of tissue growth and signalling)
Reporting period: 2016-01-01 to 2017-12-31
The results from this fellowship have expanded our understanding of how forces drive precision of tissue development. Several signaling pathways activated downstream of mechanical stretch have been identified and their function in growth and tissue shape maintenance defined.
By screening fluorescently tagged fly reporters with a novel stretching and compression device we have established that Myosin 2 (MyoII) becomes polarized with mechanical stretch (Fig. 1). We have identified the upstream mediators of MyoII polarity through targeted RNAi approach and defined their physiological relevance in regulation of wing disc shape. By combining stretching assays and genetic mosaic techniques in Drosophila wing disc we have shown that upon loss of polarized MyoII tissue loses its stiffness and elasticity and acquires an aberrant shape. Finally we have demonstrated that polarity of MyoII changes material properties of the tissue to protect any occurrent injury from propagating and compromising tissue shape and integrity. In its entirety we have discovered a completely new molecular pathway that is essential for tissues to rapidly respond to mechanical stress to maintain a functional tissue shape. These findings are currently under review for publication.
Objective 2: Coupling of growth and mechanics in 3D tissue sculpting and fold formation
The work from this fellowship has unravelled the nature of mechanochemical coupling in the context of a developing tissue, specifically in the formation of tissue 3D folds. Based on tissue size and shape quantification, we have determined that Drosophila wing disc’s folds always formed at stereotypical position during development and thus their formation appeared to be tightly regulated. By experimentally deriving the spatial and temporal patterns of wing disc growth and coupling it into finite element simulations we have found that precise fold position is driven by mechanical stresses resultant from heterogeneous proliferation rates of the wing disc. Further in silico and experimental growth perturbation will identify key regions of growth driving tissue folding. A manuscript summarizing this work is currently being prepared.
At the Institute level and beyond this fellowship has brought multiple collaborations and new ongoing projects as well as wide range of novel mechanobiological (tissue stretching, compression, tissue force relaxation assays, tissue micropipetting) and computational (3D finite element model of the tissue) assays and techniques that will establish a unique tissue and cell mechanobiology recognition to the Host Institute. New members of Institute staff have also been hired to follow up on the findings stemming from this fellowship. Finally, given the resultant data, the fellowship will likely help the Project Lead as well as the Beneficiary to establish themselves as the to-go experts in the field of tissue mechanobiology.