Hyaluronan-rich matrices crosslinked with collagen-like peptides for 3D culture of ovarian cancer cells

Projektinformationen

MHAtriCell

ID Finanzhilfevereinbarung: 658351

DOI 10.3030/658351

Abgeschlossenes Projekt

EK-Unterschriftsdatum 20 März 2015

Startdatum 15 Juni 2015

Enddatum 14 Juni 2017

Finanziert unter

• EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions

Gesamtkosten € 183 454,80

EU-Beitrag € 183 454,80

Koordiniert durch

QUEEN MARY UNIVERSITY OF LONDON
United Kingdom

Ziel

In recent years creation of artificial matrices from relatively simple biomaterials that can support tumour growth in 3D is becoming an increasingly popular platform for recreating the tumour microenvironment (TME) in vitro since 3D cell culture models can more realistically reflect the in vivo TME compared with 2D monolayer cultures. In this project, we will design and fabricate novel hydrogel matrices comprising of hyaluronan crosslinked with collagen-like triple helical peptide amphiphiles (PAs). The novel HA-PA matrix will contain built-in cell adhesion sequence, HA-binding motif and matrix metalloproteinase (MMP) cleavable sequences. We will characterise these 3D hydrogel matrices to support the encapsulation and culture of ovarian cancer cells. The MMP-assisted hydrogel degradation, migration and invasion of ovarian cancer will be studied on these models using advanced electron microscopic techniques and enzyme assays. In the final phase of our study we will characterise the inhibitory potency of a highly potent and selector inhibitor of MMP-2 and MMP-9 on this system. The findings from this study will have direct implications in understanding the mechanisms associated with ovarian cancer dissemination and the newly developed hydrogel matrix can find further use as a novel biomaterial in tissue engineering and as a 3D cell culture model in cancer drug discovery and delivery.

Wissenschaftliches Gebiet

• medical and health sciences › basic medicine › pharmacology and pharmacy › drug discovery
• medical and health sciences › medical biotechnology › tissue engineering
• natural sciences › physical sciences › optics › microscopy › electron microscopy
• medical and health sciences › clinical medicine › oncology
• engineering and technology › industrial biotechnology › biomaterials

Programm/Programme

• H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions Main Programme
• H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility

Thema/Themen

• MSCA-IF-2014-EF - Marie Skłodowska-Curie Individual Fellowships (IF-EF)

Aufforderung zur Vorschlagseinreichung

H2020-MSCA-IF-2014

Finanzierungsplan

MSCA-IF-EF-ST - Standard EF

Koordinator

QUEEN MARY UNIVERSITY OF LONDON

• Netto-EU-Beitrag: € 183 454,80
• Adresse: 327 MILE END ROAD ; E1 4NS London ; Vereinigtes Königreich
• Region: London › Inner London — East › Tower Hamlets
• Aktivitätstyp: Higher or Secondary Education Establishments
• Gesamtkosten: € 183 454,80