Objective Over the last decades, multiple arenaviruses have emerged as zoonotic human pathogens. They cross the species barrier from their natural rodent hosts to humans and cause severe human disease. A key determinant of viral zoonotic transmission is the ability to bind the receptor molecules displayed on the surface of the host cell. Despite the threat these viruses cause to public health, little is known of how they infect the host and how they are targeted by the immune response. Furthermore, the treatment and prevention options are currently extremely limited. Using techniques in structural biology, virology, immunology and cell biology, the proposed work aims at demonstrating the molecular mechanisms by which arenaviruses attach to human cells and are neutralized by the humoral immune response. First, crystallographic analysis of viral attachment glycoproteins alone and in complex with functional cellular receptors will be performed. Second, recombinantly derived viral glycoproteins will be used as immunogens for generation of specific monoclonal antibody libraries. The co-crystal structures of viral glycoproteins with cross-reactive, neutralizing antibodies, carefully selected using cell-based assays, will reveal the structural basis of antibody-mediated arenavirus neutralization. Therefore, by analogy to the development of antibody-based therapy against Ebola virus, the outlined multidisciplinary investigation will enable the rational design of antiviral reagents and vaccines. The cross-disciplinary expertise, training and infrastructure available in the host and partner organizations will allow the Researcher to establish herself as a highly-skilled expert in molecular and structural virology. As an independent, multifaceted scientist, she will be able to make a tangible contribution to the development of novel antiviral therapies, thus improving the public health and economy and adding to science base both in Europe and worldwide. Fields of science natural sciencesbiological sciencesmicrobiologyvirologymedical and health scienceshealth sciencesinfectious diseasesRNA virusesebolamedical and health sciencesbasic medicinepharmacology and pharmacypharmaceutical drugsvaccinesmedical and health scienceshealth sciencespublic healthepidemiologyzoonosismedical and health sciencesbasic medicinepharmacology and pharmacypharmaceutical drugsantivirals Programme(s) H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions Main Programme H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility Topic(s) MSCA-IF-2014-EF - Marie Skłodowska-Curie Individual Fellowships (IF-EF) Call for proposal H2020-MSCA-IF-2014 See other projects for this call Funding Scheme MSCA-IF-EF-ST - Standard EF Coordinator THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD Net EU contribution € 183 454,80 Address WELLINGTON SQUARE UNIVERSITY OFFICES OX1 2JD Oxford United Kingdom See on map Region South East (England) Berkshire, Buckinghamshire and Oxfordshire Oxfordshire Activity type Higher or Secondary Education Establishments Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Total cost € 183 454,80
