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Multifunctional luminescent and magnetic nanoparticles for advanced biomedical applications

Periodic Reporting for period 1 - LUMIMAGNET-NANO (Multifunctional luminescent and magnetic nanoparticles for advanced biomedical applications)

Reporting period: 2015-05-18 to 2017-05-17

The Lumimagnet-Nano research project has been developed at the University of Basque Country, under the supervisioin of Prof. Malú Fernández-Gubieda, and in collaboration with international colleagues, particularly the Department of Physics, University of South Florida (USA). The main objectives of this project were:

- Synthesis and characterization of MxFe(3-x)O4 (M=Mn, Co, Ni, Zn) ferrite nanoparticles synthetized by two different approaches: a chemical route and biomineralization by magnetotactic bacteria.
- Functionalization of the magnetic nanoparticles to incorporate luminescent functionalities.
- Study of the heating properties of the magnetic nanoparticles when they are exposed to an alternating magnetic field, and potential application in magnetic hyperthermia.
"The project was terminated on the 17th of November 2015, because the fellow Rosa Martín Rodríguez got an Assistant professor position at the University of Cantabria. However, the collaboration between the fellow and the host group at the University of Basque Country is still active.

The research performed in this six months within the Lumimagnet Nano project include:

- Synthesis of monodisperse doped MxFe(3-x)O4 ferrite nanoparticles by a high-temperature solution phase reaction of Fe, Mn, Ni or Zn precursors in the presence of oleic acid and oleylamine.
- Culture of Magnetospirillum gryphiswaldense bacteria and production of Fe3O4 magnetosomes doped with Co, Ni and Mn.
- Characterization of the magnetic nanoparticles by transmission electron microscopy, vibrating sample magnetometry and infrared spectroscopy.
- In-vitro study of the cytotoxicity of the magnetosomes and their application for magnetic hypertermia.

The main results achieved so far are:

- We demonstrate that in-vitro hypertermia treatment with magnetosomes cause efficient viability decrease and growth inhibition in ANA-1 macrophage cells. We are currently writing a paper with these results which will be submitted soon for publication.
- The results obtained in collaboration with the University of South Florida about the heating efficiency in multi-functional manganese ferrite/graphene oxide nanostructures have just been accepted for publication as a paper in the journal Nanotechnology.

- We submitted a proposal entitled ""X-ray absorption near edge structure (XANES) study on monodisperse MxFe3-xO4 nanoparticles obtained by synthetic and biological routes"" to both ALBA and ESRF synchrotron facilities. We have been granted with 15 shifts of beamtime in ALBA (9-14 February 2016) and 12 shifs of beamtime in ESRF (6-10 July 2016). The XANES experiments at the K-edge of both Fe and dopant M ions will provide information not only on the oxidation state of Mn, Fe, Co, Ni and Zn ions, but also on their local environment, particularly on the site symmetry. This characterization will be extremely helpful from both fundamental and applied points of view."
The research carried out during the Lumimagnet-Nano project addresses a world-wide societal challenge in health: Development of new materials for cancer treatment. Besides, my project has helped to continue a translational research line at the host institution, previously initiated by Profs. Malu Fernández-Gubieda and Alicia Muela.

The fellow collaborated with a presentation at the Science exhibition week (Semana de la Ciencia) held in Bilbao in November 2015.
Magnetosomes internalized in ANA-1 macrophages; Hyperthermia effect on ANA-1; SAR/f for magnetosomes