Objetivo Plants synthesize a wide range of secondary metabolites, among them, terpenes. These molecules are notably produced in trichomes which are specialized structures present on the surface of aerial organs. Although the terpene biosynthetic pathways have been well deciphered, their transport across membranes remains poorly understood. This is for instance the case for sesquiterpenes such as artemisinin, an extremely potent anti-malaria drug. Despite the immense benefits of these compounds for plant defenses as well as for human health, no sesquiterpene transporters have yet been identified.It was recently shown that Pleiotropic Drug Resistance (PDR) transporters, which belong to the large ATP-Binding Cassette family, are involved in diterpene transport in Nicotiana tabacum trichomes. Thus, other PDR transporters could transport other types of terpenes such as sesquiterpenes.Recently, two PDR transporters of Artemisia annua, namely AaPDR1 and AaPDR2, have been shown to be specifically expressed in glandular and T-shaped trichomes, respectively. Interestingly, glandular trichomes are known to produce artemisinin while T-shaped trichomes have been shown to produce ß-caryophyllene. Thus, we can hypothesize that AaPDR1 and AaPDR2 transport dihydroartemisinic acid, the artemisinin precursor, and ß-caryophyllene, respectively.In order to examine this hypothesis, this project aims to express these transporters in N. tabacum BY2 cells and characterize their activity using toxicity and transport assays in whole cells as well as direct transport in plasma membrane vesicles. In case our hypothesis concerning the substrates is incorrect, we will rely on a transportomics approach, which consists of running transport assays using metabolites extracted from isolated trichomes as putative substrates. In parallel, as a side project, isolated glandular and T-shaped trichomes will be submitted to quantitative proteomic comparison in order to identify their respective metabolisms. Ámbito científico natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsproteomicsnatural sciencesbiological sciencesbiochemistrybiomoleculeslipidsnatural scienceschemical sciencescatalysisnatural scienceschemical sciencesanalytical chemistrymass spectrometrynatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsenzymes Programa(s) H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions Main Programme H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility Tema(s) MSCA-IF-2014-EF - Marie Skłodowska-Curie Individual Fellowships (IF-EF) Convocatoria de propuestas H2020-MSCA-IF-2014 Consulte otros proyectos de esta convocatoria Régimen de financiación MSCA-IF-EF-ST - Standard EF Coordinador UNIVERSITE CATHOLIQUE DE LOUVAIN Aportación neta de la UEn € 160 800,00 Dirección PLACE DE L UNIVERSITE 1 1348 Louvain La Neuve Bélgica Ver en el mapa Región Région wallonne Prov. Brabant Wallon Arr. Nivelles Tipo de actividad Higher or Secondary Education Establishments Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Participación en los programas de I+D de la UE Opens in new window Red de colaboración de HORIZON Opens in new window Coste total € 160 800,00