CORDIS
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CORDIS

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Building up a brain: understanding how neural stem cell fate and regulation controls nervous tissue architecture

Project information

Grant agreement ID: 649117

Status

Ongoing project

  • Start date

    1 July 2015

  • End date

    30 June 2020

Funded under:

H2020-EU.1.1.

  • Overall budget:

    € 1 929 713

  • EU contribution

    € 1 929 713

Hosted by:

SORBONNE UNIVERSITE

France

Objective

The brain is an extraordinary complex assembly of neuronal and glial cells that underpins cognitive functions. How adequate numbers of these cells are generated by neural stem cells in embryonic and early postnatal development and how they distribute and interconnect within brain tissue is still debated. In particular, the potentialities of individual neural stem cells, their potential heterogeneity and the mechanisms regulating their function are still poorly characterized in situ; likewise, the clonal architecture of mature brain tissue and its influence on neural circuitry are only partially explored. Deciphering these aspects is essential to link neural circuit development, structure and function, and to understand the aetiology of neurodevelopmental disorders.

We have recently established transgenic strategies to simultaneously track the lineage of multiple individual neural stem cells in the intact developing brain and experimentally perturb their development. We will use these approaches in combination with recent large-volume imaging methods for high-throughput analysis of individual neural and glial clones in the mouse cortex. This will allow us to assay neural progenitor potentialities and equivalence, characterize developmental changes occurring in the neurogenic niche, describe the clonal organization of the mature cortex and study its link with neural connectivity. To decipher intrinsic and extrinsic mechanisms regulating neural progenitor activity and understand how they produce appropriate numbers of cells, we will assay the outcome of functional perturbations targeting key steps of neural development, introduced in precursors or in their local environment. These experiments will reveal how neural stem cell output might be regulated by cell interactions and intercellular signals. This multidisciplinary project will set the basis for quantitative analysis of brain development with single-cell resolution in normal and pathological conditions.

Host institution

SORBONNE UNIVERSITE

Address

21 Rue De L'Ecole De Medecine
75006 Paris

France

Activity type

Higher or Secondary Education Establishments

EU Contribution

€ 1 929 713

UNIVERSITE PIERRE ET MARIE CURIE - PARIS 6

Address

Place Jussieu 4
75252 Paris

France

Activity type

Higher or Secondary Education Establishments

Beneficiaries (2)

UNIVERSITE PIERRE ET MARIE CURIE - PARIS 6

France

SORBONNE UNIVERSITE

France

EU Contribution

€ 1 929 713

Project information

Grant agreement ID: 649117

Status

Ongoing project

  • Start date

    1 July 2015

  • End date

    30 June 2020

Funded under:

H2020-EU.1.1.

  • Overall budget:

    € 1 929 713

  • EU contribution

    € 1 929 713

Hosted by:

SORBONNE UNIVERSITE

France