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CellViewer: super-resolution systems microscopy to assess pluripotency and differentiation of stem cells at single cell level

CellViewer: super-resolution systems microscopy to assess pluripotency and differentiation of stem cells at single cell level

Objective

In this ambitious and multi-disciplinary proposal, we aim to develop new technologies that will allow us to visualize in single cells, in parallel and at the systems level, DNA, mRNAs and proteins with nanoscale resolution. We will refer to these novel technologies as the ‘CellViewer’: a unique cutting-edge high-throughput super-resolution (SR) microscopy approach (including new hardware and software development) to collect at high-resolution a large amount of spatial and dynamic information in single cells. ‘CellViewer’ will allow us to study the mechanisms of mouse embryonic stem cell (mESC) self-renewal and differentiation upon application of specific stimuli, as a specific test case. We will analyse in single cells with high throughput, DNA remodelling at multiple specific gene loci and their corresponding production, distribution and kinetics of mRNA and protein products. We will collect a large amount of dynamic and nanoscale spatial information that will lead us to build predictive models of the phenotypic output from specific input stimuli. In turn, we will be able to develop a mechanistic understanding of how mESCs maintain their stemness or commit to differentiation. The partners of CellViewer are internationally recognized experts from academia and industry in the fields of stem cell and chromatin biology, super-resolution microscopy, quantitative modelling of biological systems, and hardware and software development. This team as a whole is uniquely suited to bring Systems Biology into the era of single cell analysis, which will be a paradigm shift in the way cellular systems will be studied.
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Coordinator

FUNDACIO CENTRE DE REGULACIO GENOMICA

Address

Carrer Doctor Aiguader 88
08003 Barcelona

Spain

Activity type

Research Organisations

EU Contribution

€ 2 086 668,26

Participants (5)

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FUNDACIO INSTITUT DE CIENCIES FOTONIQUES

Spain

EU Contribution

€ 293 334,24

EUROPEAN MOLECULAR BIOLOGY LABORATORY

Germany

THE HEBREW UNIVERSITY OF JERUSALEM

Israel

EU Contribution

€ 792 500

LABORATORY IMAGING SRO

Czechia

EU Contribution

€ 128 750

Idea Bio-Medical Ltd.

Israel

EU Contribution

€ 687 500

Project information

Grant agreement ID: 686637

Status

Ongoing project

  • Start date

    1 February 2016

  • End date

    31 January 2020

Funded under:

H2020-EU.1.2.1.

  • Overall budget:

    € 3 988 752,50

  • EU contribution

    € 3 988 752,50

Coordinated by:

FUNDACIO CENTRE DE REGULACIO GENOMICA

Spain