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CellViewer: super-resolution systems microscopy to assess pluripotency and differentiation of stem cells at single cell level

Objective

In this ambitious and multi-disciplinary proposal, we aim to develop new technologies that will allow us to visualize in single cells, in parallel and at the systems level, DNA, mRNAs and proteins with nanoscale resolution. We will refer to these novel technologies as the ‘CellViewer’: a unique cutting-edge high-throughput super-resolution (SR) microscopy approach (including new hardware and software development) to collect at high-resolution a large amount of spatial and dynamic information in single cells. ‘CellViewer’ will allow us to study the mechanisms of mouse embryonic stem cell (mESC) self-renewal and differentiation upon application of specific stimuli, as a specific test case. We will analyse in single cells with high throughput, DNA remodelling at multiple specific gene loci and their corresponding production, distribution and kinetics of mRNA and protein products. We will collect a large amount of dynamic and nanoscale spatial information that will lead us to build predictive models of the phenotypic output from specific input stimuli. In turn, we will be able to develop a mechanistic understanding of how mESCs maintain their stemness or commit to differentiation. The partners of CellViewer are internationally recognized experts from academia and industry in the fields of stem cell and chromatin biology, super-resolution microscopy, quantitative modelling of biological systems, and hardware and software development. This team as a whole is uniquely suited to bring Systems Biology into the era of single cell analysis, which will be a paradigm shift in the way cellular systems will be studied.

Call for proposal

H2020-FETOPEN-2014-2015-RIA
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Coordinator

FUNDACIO CENTRE DE REGULACIO GENOMICA
Address
Carrer Doctor Aiguader 88
08003 Barcelona
Spain
Activity type
Research Organisations
EU contribution
€ 2 086 668,26

Participants (5)

FUNDACIO INSTITUT DE CIENCIES FOTONIQUES

Participation ended

Spain
EU contribution
€ 293 334,24
Address
Avinguda Carl Friedrich Gauss 3
08860 Castelldefels
Activity type
Research Organisations
EUROPEAN MOLECULAR BIOLOGY LABORATORY

Participation ended

Germany
EU contribution
€ 0
Address
Meyerhofstrasse 1
69117 Heidelberg
Activity type
Research Organisations
THE HEBREW UNIVERSITY OF JERUSALEM
Israel
EU contribution
€ 792 500
Address
Edmond J Safra Campus Givat Ram
91904 Jerusalem
Activity type
Higher or Secondary Education Establishments
LABORATORY IMAGING SRO
Czechia
EU contribution
€ 128 750
Address
Za Drahou 171/17
102 00 Praha
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Idea Bio-Medical Ltd.
Israel
EU contribution
€ 687 500
Address
Prof. Bergman St. 2
76705 Rehovot
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)