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A portable MicroNanoBioSystem and Instrument for ultra-fast analysis of pathogens in food: Innovation from LOVE-FOOD lab prototype to a pre-commercial instrument

Objective

The development of new methodologies advancing the state of the art in foodborne pathogen detection is a challenge for scientists and technologists as well as food industry and consumers. This project aims to meet the challenge by providing a reliable and versatile solution thanks to the convergence of micro-nano-bio systems. The work capitalizes on several innovative concepts which have already been proven to meet the required criteria for fast, low cost and highly sensitive analysis of pathogens in food samples in a previous research project entitled LoveFood.

These concepts are gathered on a credit-card size Lab-on-Chip platform, where all necessary steps for bacteria detection are performed on several chips. Specifically, bacteria capture and lysis (one chip), DNA extraction (second chip) and amplification (third chip) and finally pathogenic-DNA detection (fourth chip) can be performed in less than 8 hours and without the need for skilled personnel or large, lab-based dedicated equipment.

To proceed for a higher Technology Readiness Level towards the successful commercialization of the current prototype and produce a portable, and rapid platform (targeting total pathogen analysis time 4 hours including a 3 hour pre-enrichment step), we propose to further develop it by integrating the bacteria lysis, DNA purification and amplification modules, as well as the biochip detection platform on a single cartridge, able to perform multi-pathogen analysis (i.e. Salmonella, Listeria, Escherichia coli and Bacillus cereus) in multiple samples. The system will be developed for dairy products and meat analysis, with a strong commitment to produce a pre-industrial prototype by the end of the project.

Coordinator

IDRYMA TECHNOLOGIAS KAI EREVNAS

Address

N Plastira Str 100
70013 Irakleio

Greece

Activity type

Research Organisations

EU Contribution

€ 809 791,25

Participants (6)

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SENSEOR SAS

France

EU Contribution

€ 394 875

JOBST TECHNOLOGIES GMBH

Germany

EU Contribution

€ 316 750

"NATIONAL CENTER FOR SCIENTIFIC RESEARCH ""DEMOKRITOS"""

Greece

EU Contribution

€ 994 833,75

UNIVERZITA PARDUBICE

Czechia

EU Contribution

€ 253 500

INSTITUT PASTEUR

France

EU Contribution

€ 340 000

MAKEDONIKI VIOMIHANIA GALAKTOS ANONIMOS ETERIA (MEVGAL SA)

Greece

EU Contribution

€ 42 000

Project information

Grant agreement ID: 687681

Status

Closed project

  • Start date

    1 February 2016

  • End date

    30 April 2019

Funded under:

H2020-EU.2.1.1.

  • Overall budget:

    € 3 474 732,50

  • EU contribution

    € 3 151 750

Coordinated by:

IDRYMA TECHNOLOGIAS KAI EREVNAS

Greece