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A portable MicroNanoBioSystem and Instrument for ultra-fast analysis of pathogens in food: Innovation from LOVE-FOOD lab prototype to a pre-commercial instrument

Objective

The development of new methodologies advancing the state of the art in foodborne pathogen detection is a challenge for scientists and technologists as well as food industry and consumers. This project aims to meet the challenge by providing a reliable and versatile solution thanks to the convergence of micro-nano-bio systems. The work capitalizes on several innovative concepts which have already been proven to meet the required criteria for fast, low cost and highly sensitive analysis of pathogens in food samples in a previous research project entitled LoveFood.

These concepts are gathered on a credit-card size Lab-on-Chip platform, where all necessary steps for bacteria detection are performed on several chips. Specifically, bacteria capture and lysis (one chip), DNA extraction (second chip) and amplification (third chip) and finally pathogenic-DNA detection (fourth chip) can be performed in less than 8 hours and without the need for skilled personnel or large, lab-based dedicated equipment.

To proceed for a higher Technology Readiness Level towards the successful commercialization of the current prototype and produce a portable, and rapid platform (targeting total pathogen analysis time 4 hours including a 3 hour pre-enrichment step), we propose to further develop it by integrating the bacteria lysis, DNA purification and amplification modules, as well as the biochip detection platform on a single cartridge, able to perform multi-pathogen analysis (i.e. Salmonella, Listeria, Escherichia coli and Bacillus cereus) in multiple samples. The system will be developed for dairy products and meat analysis, with a strong commitment to produce a pre-industrial prototype by the end of the project.

Call for proposal

H2020-ICT-2015
See other projects for this call

Coordinator

IDRYMA TECHNOLOGIAS KAI EREVNAS
Address
N Plastira Str 100
70013 Irakleio
Greece
Activity type
Research Organisations
EU contribution
€ 809 791,25

Participants (6)

SENSEOR SAS
France
EU contribution
€ 394 875
Address
505 Route Des Lucioles Le Navigator Bat B Parc Du Font De L Orme Lot 3
06560 Valbonne
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
JOBST TECHNOLOGIES GMBH
Germany
EU contribution
€ 316 750
Address
Engesserstrasse 4 B
79108 Freiburg
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
"NATIONAL CENTER FOR SCIENTIFIC RESEARCH ""DEMOKRITOS"""
Greece
EU contribution
€ 994 833,75
Address
End Of Patriarchou Grigoriou E And 27 Neapoleos Street
15341 Agia Paraskevi
Activity type
Research Organisations
UNIVERZITA PARDUBICE
Czechia
EU contribution
€ 253 500
Address
Studentska 95
532 10 Pardubice
Activity type
Higher or Secondary Education Establishments
INSTITUT PASTEUR
France
EU contribution
€ 340 000
Address
Rue Du Docteur Roux 25-28
75724 Paris Cedex 15
Activity type
Research Organisations
MAKEDONIKI VIOMIHANIA GALAKTOS ANONIMOS ETERIA (MEVGAL SA)
Greece
EU contribution
€ 42 000
Address
Koyfalia
57100 Koyfalia, Thessaloniki
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)