Objectif Epithelial barriers protect the body against physical, chemical, and microbial insults. Intestinal epithelium is one of the most actively renewing tissues in the body and a major site of carcinogenesis. Functional in vitro models of intestinal epithelium have been pursued for a long time. They are key elements in basic research, disease modelling, drug discovery, and tissue replacing and have become prime models for adult stem cell research. By taking advantage of the self-organizing properties of intestinal stem cells, intestinal organoids have been recently established, showing cell renewal’s kinetics resembling to the one found in vivo. However, the development of in vitro 3D tissue equivalents accounting for the dimensions, architecture and access to the luminal contents of the in vivo human intestinal tissue together with its self-renewal properties and cell complexity, remains a challenge. The goal of this project is to engineer intestinal epithelial tissue models that mimic physiological characteristics found in in vivo human intestinal tissue, to open up new areas of research on human intestinal diseases. The proposed models will address the in vivo intestinal epithelial cell renewal and migration, the multicell-type differentiation and the epithelial cell interactions with the underlying basement membrane while providing access to the luminal content to go beyond the state-of-the-art organoid models. To do this, we propose to develop an experimental setup that combines microfabrication techniques, tissue engineering components and recent advances in intestinal stem cell research, exploiting stem cell self-organizing characteristics. We anticipate this setup to recapitulate the 3D morphology, the spatio-chemical gradients and the dynamic microenvironment of the living tissue. We expect the new device to prove useful in understanding cell physiology, adult stem cell behaviour, and organ development as well as in modelling human intestinal diseases. Champ scientifique medical and health sciencesbasic medicinepharmacology and pharmacydrug discoverymedical and health sciencesclinical medicinegastroenterologyinflammatory bowel diseasemedical and health sciencesmedical biotechnologytissue engineeringmedical and health sciencesmedical biotechnologycells technologiesstem cellsmedical and health sciencesclinical medicinecardiologycardiovascular diseases Programme(s) H2020-EU.1.1. - EXCELLENT SCIENCE - European Research Council (ERC) Main Programme Thème(s) ERC-CoG-2014 - ERC Consolidator Grant Appel à propositions ERC-2014-CoG Voir d’autres projets de cet appel Régime de financement ERC-COG - Consolidator Grant Institution d’accueil FUNDACIO INSTITUT DE BIOENGINYERIA DE CATALUNYA Contribution nette de l'UE € 1 997 190,00 Adresse CARRER BALDIRI REIXAC PLANTA 2A 10-12 08028 Barcelona Espagne Voir sur la carte Région Este Cataluña Barcelona Type d’activité Research Organisations Liens Contacter l’organisation Opens in new window Site web Opens in new window Participation aux programmes de R&I de l'UE Opens in new window Réseau de collaboration HORIZON Opens in new window Coût total € 1 997 190,00 Bénéficiaires (1) Trier par ordre alphabétique Trier par contribution nette de l'UE Tout développer Tout réduire FUNDACIO INSTITUT DE BIOENGINYERIA DE CATALUNYA Espagne Contribution nette de l'UE € 1 997 190,00 Adresse CARRER BALDIRI REIXAC PLANTA 2A 10-12 08028 Barcelona Voir sur la carte Région Este Cataluña Barcelona Type d’activité Research Organisations Liens Contacter l’organisation Opens in new window Site web Opens in new window Participation aux programmes de R&I de l'UE Opens in new window Réseau de collaboration HORIZON Opens in new window Coût total € 1 997 190,00