Periodic Reporting for period 2 - SATBIOFUN (Genome editing for spatiotemporal analysis of centriolar SATellite BIOgenesis and FUNction in cellular stress responses)
Reporting period: 2018-07-01 to 2019-06-30
The central scientific goal of this project was to expand our knowledge with regard to satellite function and contribution to maintaining a disease-free state. The realization of this goal was achieved through a number of specific objectives, namely: the generation of a unique toolbox of reagents with which to study satellites; profiling of satellite protein contribution to normal satellite function; and the definition of the contribution of satellites to cell stress responses. Furthering our understanding of satellites function enhances our knowledge of how their dysregulation may lead to disease. To achieve these objectives, we used genome-editing and cutting-edge imaging techniques. This allowed us to define a novel function for satellites in the cellular response to proteostatic stress, thereby identifying a previously unexplored link between the centrosome and proteostasis fields.
In realizing our objectives, the Fellow received extensive training in a range of state-of-the-art techniques during an Outgoing Phase in Canada. The Return Phase in Ireland allowed the transfer of this knowledge to Europe, and in doing so enhanced the European research environment. The Fellow also acquired a range of complementary skills, allowing the fulfillment of the overarching objective of developing the Fellow’s career to a position of professional maturity and facilitating the goal of establishing her own independent research group.