Periodic Reporting for period 1 - ADAPTIVE INVERSIONS (Chromosomes on shuffle: disentangling the relative contributions of natural selection, sexual selection, and drift on the evolution of a chromosomal inversion.)
Reporting period: 2016-05-01 to 2018-04-30
1. Conducted a large-scale whole genome re-sequencing study (60 genomes sequenced) to examine diversity and divergence within the inversion and to look for signatures of natural selection. This data revealed that α and β are highly divergent and that they contain more population specific variation than expected by chance. Re-analysis of this data using an updated genome assembly is currently ongoing. Together with colleagues we also developed a SNP marker for the inversion. This paper is published in Proceedings of the Royal Society B: https://doi.org/10.1098/rspb.2018.0519.
2. Examined a potential driver of adaptation, the microbiome of the wrackbed (the piles of rotting seaweed where C. frigida lives, and the sole food source for C. frigida larvae) in depth. We sequenced 16S DNA from both the wrackbed microbiome and the larval gut microbiome from 5 different populations across Scandinavia. This data revealed that wrackbed microbiomes are highly variable between populations but consistent within populations. The salinity of the surrounding water is a major factor driving this variation. Larval gut microbiomes were also highly variable but not related to the inversion genotype. This manuscript is currently in preparation.
3. Detailed the link between local environment, selection pressures on the inversion, and phenotypic effects of the inversion. We conducted a balanced reciprocal transplant experiment where C. frigida larvae were from 4 populations were raised on 4 substrates (seaweed) from all 4 populations. We examined changes in the frequencies of the karyotypes across populations and substrates as well as shifts in two phenotypes (body size and development time) known to be associated with the inversion. The results from this study indicated found that local environment strongly modified karyotype frequencies and that this effect differed between populations. Inversion-related phenotypes showed stable patterns but the magnitude of differences between groups was affected by population and substrate. The manuscript is currently in review and available on bioRxiv (https://doi.org/10.1101/295568).
4. Elucidated a signal for sexual selection in C. frigida (cuticular hydrocarbons) and investigated links between this signal, environmental conditions, and the inversion. Natural and sexual selection interact when traits are under both natural and sexual selection or when sexually selected traits are influenced by environmental conditions. We were interested in the signal used for sexual selection in C. frigida and if that signal could be linked to the inversion, the environment, or both. Cuticular hydrocarbons (CHCs) are often used as chemical signals in insects so we designed a study to: 1. Describe the CHC composition of C. frigida, 2. Determine the variance caused by population, diet, sex, and the inversion. We found significant effects of population, sex, diet, and the inversion. This suggests that CHCs are used as sexual signals in C. frigida and that they potentially represent a major intersection of natural and sexual selection. This manuscript is currently in review and can be found on bioRxiv (https://doi.org/10.1101/303206
5. Completed labwork for a gene expression study to determine the role of gene expression in inversion heterosis. The funding for RNA-sequencing has been secured and samples will be sequenced Summer/Fall 2018.
Results from this project have been presented at three international conferences with a third presentation scheduled for August 2018. One manuscript has been accepted, two are in review, and two are in preparation. We expect five other manuscripts to be generated from this work making a minimum total of nine. Significant engagement with the public has taken place via presentations of the project and C. frigida system to groups visiting Gothenburg University, the establishment of a photo contest bringing together scientists and the public, and media coverage of the project (two separate interviews with Swedish Radio).
It is hoped that the ecological aspects of this work draw attention to the wrackbed environment as one of extreme importance and can be used to influence EU strategy for sustainable development. Coelopa frigida is one of few organisms that link marine and terrestrial habitats as their food source is marine bacteria that grow on macroalgae and they are prey items for shorebirds. Given the unique niche they occupy, they are potentially an important target for assessing ecosystem health.