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Novel approaches to determining the function of tissue-specific regulatory T cells

Objective

Regulatory T cells (Tregs) are formed through the expression of the transcription factor Foxp3 in T cells, resulting in the rewiring of the cell function into an immunosuppressive phenotype. Recently, it has been proposed that Tregs also have additional tissue-specific physiological roles when resident in different tissues. For example, tissue-specific Tregs residing in the muscle and adipose tissue possess immunological and non-immunological functions in these tissues, distinct from the generic Tregs in circulation. Currently, research into tissue-specific functions of Tregs, or any other migratory cell type, is limited by the available research tools. A vital part of immunological studies is cell depletion, yet a major limitation of all available methods is that they deplete target cells across the entire organism. This makes it extremely difficult to ascertain the function of tissue-resident Tregs, as systemic deletion results in severe autoimmunity, confounding the study of tissue-specific subsets. In order to assess these tissue-resident subsets new research tools are required to deplete the target cells in a specific anatomical region while leaving the same cell type unaffected in other organs. This project proposes to generate new synthetic biology tools for depleting tissue-resident cells and then to apply these tools to the study of tissue-resident Tregs in the brain, lung, liver, kidney and pancreas, thus creating a comprehensive atlas of tissue-specific functions. These studies will be extended by systematic molecular, cellular and kinetic analysis using existing innovative methods established in the laboratory. Finally, our tissue-specific deletion system will have a profound impact on immunology beyond the direct scope of the project, as the tools will be developed to allow flexible application to any cell type. In essence, this is a field of research currently held back by the absence of appropriate tools, waiting for the generation of a new toolkit.

Call for proposal

ERC-2015-CoG
See other projects for this call

Host institution

THE BABRAHAM INSTITUTE
Address
Babraham Hall
CB22 3AT Cambridge
United Kingdom
Activity type
Research Organisations
EU contribution
€ 888 793,41

Beneficiaries (2)

THE BABRAHAM INSTITUTE
United Kingdom
EU contribution
€ 888 793,41
Address
Babraham Hall
CB22 3AT Cambridge
Activity type
Research Organisations
VIB VZW

Participation ended

Belgium
EU contribution
€ 1 110 741,59
Address
Rijvisschestraat 120
9052 Zwijnaarde - Gent
Activity type
Research Organisations