Objective Small non-coding RNAs play important roles in gene expression regulation. Initially the attention was focused on the 21-24-nucleotide small RNAs such as microRNAs but later on deeper sequencing experiments using next generation sequencing revealed a slightly longer class of small RNAs that are 30-34 nucleotides long. These longer small RNAs are often generated from known non-coding RNAs such as tRNA or snoRNA. The biogenesis of these longer small RNAs seems to be diverse and is not well understood. The host laboratory have characterised the biogenesis of such longer small RNAs generated from Y RNAs in mammalian cells and found that it is different from microRNA biogenesis but also from the way tRNA derived small RNAs are produced. Results from a high-throughput mutagenesis approach suggest that the secondary structure of the Y RNA, rather than its sequence, determines where the cleavage happens that liberates the small RNAs from the 3' end of Y RNAs. This project aims validating these results and also to apply a high-throughput mutagensis screen on the 5' region of the Y RNA to study the production of small RNAs from that end of the molecule. Combining the results for Y RNA derived longer small RNA generation from both 5' and 3' end of these molecules will enable the experienced researcher to establish how this class of small RNAs are produced by designing specific novel mutants and test them in mammalian cells. Another aim of the project is to give training to the experienced researcher in mammalian cell biology and bioinformatics to add his existing molecular biology and microbiology skills. Fields of science natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsnatural sciencesbiological sciencescell biologynatural sciencesbiological sciencesgeneticsmutationnatural sciencesbiological sciencesgeneticsnucleotidesnatural sciencesbiological sciencesgeneticsRNA Programme(s) H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions Main Programme H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility Topic(s) MSCA-IF-2015-EF - Marie Skłodowska-Curie Individual Fellowships (IF-EF) Call for proposal H2020-MSCA-IF-2015 See other projects for this call Funding Scheme MSCA-IF-EF-ST - Standard EF Coordinator UNIVERSITY OF EAST ANGLIA Net EU contribution € 195 454,80 Address EARLHAM ROAD NR4 7TJ Norwich United Kingdom See on map Region East of England East Anglia Norwich and East Norfolk Activity type Higher or Secondary Education Establishments Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Total cost € 195 454,80
