CORDIS
EU research results

CORDIS

English EN
Microbial invasion and dissemination within the host, mechanisms and effects

Microbial invasion and dissemination within the host, mechanisms and effects

Objective

An infection is defined by the deleterious consequences of the interactions between a pathogen and a host. Thus, studying the biology of infection reveals critical properties of hosts and pathogens, and is a way forward to address basic biological questions and improve health.
We study listeriosis, a systemic infection caused by Listeria monocytogenes (Lm). Lm is a human foodborne pathogen that crosses the intestinal barrier, disseminates systemically, replicates in liver and spleen and reaches the central nervous system (CNS) and fetoplacental unit. Given the remarkable journey Lm makes in its host, studying listeriosis offers unprecedented opportunities to understand host cell biology, physiology and immune responses, guided by Lm. The mucosal, CNS and fetoplacental tropisms of Lm are shared by other microbes which pathogenesis is far less understood. Lm therefore stands as a unique model microorganism of general biological and medical significance.
The major challenge of this project is to go beyond reductionist approaches and embrace the complexity of actual infections.
We will use stem cell-derived organoids, live imaging, genetically engineered mouse models, the clinical and biological data from a unique cohort of 900 patients and the corresponding causative Lm strains, to investigate the molecular mechanisms of Lm tissue invasion, dissemination and host responses.
Specifically, we will (i) decipher the cell biology of microbial translocation across the intestinal epithelium; (ii) study the impact of microbial portal of entry on microbial fate, dissemination and host responses; (iii) harness Lm biodiversity to identify novel virulence factors and (iv) discover new host factors predisposing to invasive infections.
Building on the unique combination of advanced experimental systems and exclusive clinical data, this integrative and innovative project will reveal novel, physiologically relevant mechanisms of infection, with scientific and biomedical implications.
Leaflet | Map data © OpenStreetMap contributors, Credit: EC-GISCO, © EuroGeographics for the administrative boundaries

Host institution

INSTITUT PASTEUR

Address

Rue Du Docteur Roux 25-28
75724 Paris Cedex 15

France

Activity type

Higher or Secondary Education Establishments

EU Contribution

€ 2 750 000

Beneficiaries (1)

Sort alphabetically

Sort by EU Contribution

Expand all

INSTITUT PASTEUR

France

EU Contribution

€ 2 750 000

Project information

Grant agreement ID: 681829

Status

Ongoing project

  • Start date

    1 November 2016

  • End date

    31 October 2021

Funded under:

H2020-EU.1.1.

  • Overall budget:

    € 2 750 000

  • EU contribution

    € 2 750 000

Hosted by:

INSTITUT PASTEUR

France