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New enzymatic oxidation/oxyfunctionalization technologies for added value bio-based products

Objective

Sustainable production of chemical building blocks and other added value products from plant biomass is required for a bio-based economy. However, the biomass biorefineries should benefit not only from the use of renewable feedstocks but also from greener and more efficient bio-chemical technologies. Previous projects have shown the potential of oxidative enzymes in the production of some added value compounds from biomass components. Of special interest are still unexplored oxidation/oxyfunctionalization reactions (of sugar and lipid compounds) by microbial oxidoreductases, including new (self-sufficient) heme-thiolate peroxygenases. In this context, EnzOx2 plans to develop a 100% biochemical conversion of bio-based 5-hydroxymethylfurfural (HMF) into diformylfuran, a platform chemical, and 2,5-furandicarboxylic acid (FDCA), a plastic building-block. Oxidases (flavo and copper/radical) and peroxygenases will be used to perform the three-step oxidation of HMF to FDCA in a co-substrate and side-product free, one-pot conversion. On the other hand, highly (regio/stereo) selective hydroxylation of plant lipids (such as fatty acids, terpenes and steroids) by peroxygenases will be optimized for cost-effective production of flavours and fragrances (F&F), active pharmaceutical ingredients (APIs) and others. ENZOX2 aims to solve some main bottlenecks in these industrial processes by the use of bio-chemical tools (new/engineered enzymes and optimized biotransformations), to be later validated at the pilot/flagship scale. To attain this objective the consortium includes: i) two world leaders in industrial enzymes (Novozymes) and F&F (Firmenich); ii) two chemical SMEs producing HMF and chiral APIs (AVA-Biochem and Chiracon); iii) two specialized biotechnology SMEs (JenaBios and CLEA); iv) one technology centre in the Plastics sector (AIMPLAS); and v) three CSIC institutes and two universities (Dresden and Delft) with expertise in enzyme reactions and bioprocess implementation.

Call for proposal

H2020-BBI-PPP-2015-2-1
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Coordinator

AGENCIA ESTATAL CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS
Address
Calle Serrano 117
28006 Madrid
Spain
Activity type
Research Organisations
EU contribution
€ 1 306 000

Participants (9)

TECHNISCHE UNIVERSITAET DRESDEN
Germany
EU contribution
€ 450 000
Address
Helmholtzstrasse 10
01069 Dresden
Activity type
Higher or Secondary Education Establishments
JENABIOS GMBH
Germany
EU contribution
€ 244 000
Address
Lobstedter Strasse 80
07749 Jena
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
FIRMENICH SA
Switzerland
EU contribution
€ 0
Address
Route Des Jeunes 1
1227 Geneve
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
AVA BIOCHEM BSL AG
Switzerland
EU contribution
€ 0
Address
Rothausstrasse 61
4132 Muttenz
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
NOVOZYMES A/S
Denmark
EU contribution
€ 0
Address
Krogshoejvej 36
2880 Bagsvaerd
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
TECHNISCHE UNIVERSITEIT DELFT
Netherlands
EU contribution
€ 400 000
Address
Stevinweg 1
2628 CN Delft
Activity type
Higher or Secondary Education Establishments
CHIRACON GMBH
Germany
EU contribution
€ 250 000
Address
Biotechnologiepark Tgz 1
14943 Luckenwalde
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
CLEA TECHNOLOGIES BV
Netherlands
EU contribution
€ 200 000
Address
Delftechpark 34
2628 XH Delft
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
AIMPLAS - ASOCIACION DE INVESTIGACION DE MATERIALES PLASTICOS Y CONEXAS
Spain
EU contribution
€ 150 000
Address
Valencia Parc Technologic Calle Gustave Eiffel 4
46980 Paterna Valencia
Activity type
Research Organisations