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New enzymatic oxidation/oxyfunctionalization technologies for added value bio-based products

Objective

Sustainable production of chemical building blocks and other added value products from plant biomass is required for a bio-based economy. However, the biomass biorefineries should benefit not only from the use of renewable feedstocks but also from greener and more efficient bio-chemical technologies. Previous projects have shown the potential of oxidative enzymes in the production of some added value compounds from biomass components. Of special interest are still unexplored oxidation/oxyfunctionalization reactions (of sugar and lipid compounds) by microbial oxidoreductases, including new (self-sufficient) heme-thiolate peroxygenases. In this context, EnzOx2 plans to develop a 100% biochemical conversion of bio-based 5-hydroxymethylfurfural (HMF) into diformylfuran, a platform chemical, and 2,5-furandicarboxylic acid (FDCA), a plastic building-block. Oxidases (flavo and copper/radical) and peroxygenases will be used to perform the three-step oxidation of HMF to FDCA in a co-substrate and side-product free, one-pot conversion. On the other hand, highly (regio/stereo) selective hydroxylation of plant lipids (such as fatty acids, terpenes and steroids) by peroxygenases will be optimized for cost-effective production of flavours and fragrances (F&F), active pharmaceutical ingredients (APIs) and others. ENZOX2 aims to solve some main bottlenecks in these industrial processes by the use of bio-chemical tools (new/engineered enzymes and optimized biotransformations), to be later validated at the pilot/flagship scale. To attain this objective the consortium includes: i) two world leaders in industrial enzymes (Novozymes) and F&F (Firmenich); ii) two chemical SMEs producing HMF and chiral APIs (AVA-Biochem and Chiracon); iii) two specialized biotechnology SMEs (JenaBios and CLEA); iv) one technology centre in the Plastics sector (AIMPLAS); and v) three CSIC institutes and two universities (Dresden and Delft) with expertise in enzyme reactions and bioprocess implementation.

Call for proposal

H2020-BBI-PPP-2015-02

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Sub call

H2020-BBI-PPP-2015-2-1

Coordinator

AGENCIA ESTATAL CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS
Net EU contribution
€ 1 306 000,00
Address
Calle Serrano 117
28006 Madrid
Spain

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Region
Comunidad de Madrid Comunidad de Madrid Madrid
Activity type
Research Organisations
Other funding
€ 508 000,00

Participants (12)

TECHNISCHE UNIVERSITAET DRESDEN
Germany
Net EU contribution
€ 450 000,00
Address
Helmholtzstrasse 10
01069 Dresden

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Region
Sachsen Dresden Dresden, Kreisfreie Stadt
Activity type
Higher or Secondary Education Establishments
Other funding
€ 150 000,00
JENABIOS GMBH
Germany
Net EU contribution
€ 244 000,00
Address
Lobstedter Strasse 80
07749 Jena

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SME

The organization defined itself as SME (small and medium-sized enterprise) at the time the Grant Agreement was signed.

Yes
Region
Thüringen Thüringen Jena, Kreisfreie Stadt
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Other funding
€ 81 000,00
FIRMENICH SA
Switzerland
Net EU contribution
€ 0,00
Address
Route Des Jeunes 1
1227 Geneve

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Region
Schweiz/Suisse/Svizzera Région lémanique Genève
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Other funding
€ 118 750,00
AVA BIOCHEM BSL AG
Switzerland
Net EU contribution
€ 0,00
Address
Rothausstrasse 61
4132 Muttenz

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SME

The organization defined itself as SME (small and medium-sized enterprise) at the time the Grant Agreement was signed.

Yes
Region
Schweiz/Suisse/Svizzera Nordwestschweiz Basel-Landschaft
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Other funding
€ 509 000,00
Third-party

Legal entity other than a subcontractor which is affiliated or legally linked to a participant. The entity carries out work under the conditions laid down in the Grant Agreement, supplies goods or provides services for the action, but did not sign the Grant Agreement. A third party abides by the rules applicable to its related participant under the Grant Agreement with regard to eligibility of costs and control of expenditure.

AVALON INDUSTRIES AG
Switzerland
Net EU contribution
€ 0,00
Address
Baarerstrasse 20
6300 Zug

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Region
Schweiz/Suisse/Svizzera Zentralschweiz Zug
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Other funding
€ 100 000,00
Third-party

Legal entity other than a subcontractor which is affiliated or legally linked to a participant. The entity carries out work under the conditions laid down in the Grant Agreement, supplies goods or provides services for the action, but did not sign the Grant Agreement. A third party abides by the rules applicable to its related participant under the Grant Agreement with regard to eligibility of costs and control of expenditure.

AVA CO2 SCHWEIZ AG

Participation ended

Switzerland
Net EU contribution
€ 0,00
Address
Baarerstrasse 20
6300 Zug
SME

The organization defined itself as SME (small and medium-sized enterprise) at the time the Grant Agreement was signed.

Yes
Region
Schweiz/Suisse/Svizzera Zentralschweiz Zug
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Other funding
€ 0,00
NOVOZYMES A/S
Denmark
Net EU contribution
€ 0,00
Address
Krogshoejvej 36
2880 Bagsvaerd

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Region
Danmark Hovedstaden Københavns omegn
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Other funding
€ 200 000,00
TECHNISCHE UNIVERSITEIT DELFT
Netherlands
Net EU contribution
€ 400 000,00
Address
Stevinweg 1
2628 CN Delft

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Region
West-Nederland Zuid-Holland Delft en Westland
Activity type
Higher or Secondary Education Establishments
Other funding
€ 133 000,00
CHIRACON GMBH
Germany
Net EU contribution
€ 250 000,00
Address
Biotechnologiepark Tgz 1
14943 Luckenwalde

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SME

The organization defined itself as SME (small and medium-sized enterprise) at the time the Grant Agreement was signed.

Yes
Region
Brandenburg Brandenburg Teltow-Fläming
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Other funding
€ 83 000,00
CLEA TECHNOLOGIES BV

Participation ended

Netherlands
Net EU contribution
€ 0,00
Address
Delftechpark 34
2628 XH Delft
SME

The organization defined itself as SME (small and medium-sized enterprise) at the time the Grant Agreement was signed.

Yes
Region
West-Nederland Zuid-Holland Delft en Westland
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Other funding
€ 0,00
AIMPLAS - ASOCIACION DE INVESTIGACION DE MATERIALES PLASTICOS Y CONEXAS
Spain
Net EU contribution
€ 150 000,00
Address
Valencia Parc Technologic Calle Gustave Eiffel 4
46980 Paterna Valencia

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SME

The organization defined itself as SME (small and medium-sized enterprise) at the time the Grant Agreement was signed.

Yes
Region
Este Comunitat Valenciana Valencia/València
Activity type
Research Organisations
Other funding
€ 50 000,00
UNIVERSIDAD DE SANTIAGO DE COMPOSTELA
Spain
Net EU contribution
€ 200 000,00
Address
Colexio De San Xerome Praza Do Obradoiro S/n
15782 Santiago De Compostela

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Region
Galicia A Coruña
Activity type
Higher or Secondary Education Establishments
Other funding
€ 67 250,00