Chemistry and Biology in Synergy - Studies of hydrogenases using a combination of synthetic chemistry and biological tools

Projektinformationen

CaBiS

ID Finanzhilfevereinbarung: 714102

DOI 10.3030/714102

Abgeschlossenes Projekt

EK-Unterschriftsdatum 14 November 2016

Startdatum 1 Februar 2017

Enddatum 31 Januar 2022

Finanziert unter

• EXCELLENT SCIENCE - European Research Council (ERC)

Gesamtkosten € 1 494 880,00

EU-Beitrag € 1 494 880,00

Koordiniert durch

UPPSALA UNIVERSITET
Sweden

Ziel

My proposal aims to take advantage of my ground-breaking finding that it is possible to mature, or activate, the [FeFe] hydrogenase enzyme (HydA) using synthetic mimics of its catalytic [2Fe] cofactor. (Berggren et al, Nature, 2013) We will now explore the chemistry and (bio-)technological potential of the enzyme using an interdisciplinary approach ranging from in vivo biochemical studies all the way to synthetic model chemistry. Hydrogenases catalyse the interconversion between protons and H2 with remarkable efficiency. Consequently, they are intensively studied as alternatives to Pt-catalysts for these reactions, and are arguably of high (bio-) technological importance in the light of a future “hydrogen society”.
The project involves the preparation of novel “artificial” hydrogenases with the primary aim of designing spectroscopic model systems via modification(s) of the organometallic [2Fe] subsite. In parallel we will prepare in vitro loaded forms of the maturase HydF and study its interaction with apo-HydA in order to further elucidate the maturation process of HydA. Moreover we will develop the techniques necessary for in vivo application of the artificial activation concept, thereby paving the way for a multitude of studies including the reactivity of artificial hydrogenases inside a living cell, but also e.g. gain-of-function studies in combination with metabolomics and proteomics. Inspired by our work on the artificial maturation system we will also draw from our knowledge of Nature’s [FeS] cluster proteins in order to prepare a novel class of “miniaturized hydrogenases” combining synthetic [4Fe4S] binding oligopeptides with [2Fe] cofactor model compounds.
Our interdisciplinary approach is particularly appealing as it not only provides further insight into hydrogenase chemistry and the maturation of metalloproteins, but also involves the development of novel tools and concepts applicable to the wider field of bioinorganic chemistry.

Wissenschaftliches Gebiet

• natural sciences › chemical sciences › inorganic chemistry › bioinorganic chemistry
• natural sciences › biological sciences › biochemistry › biomolecules › proteins › proteomics
• natural sciences › biological sciences › biochemistry › biomolecules › proteins › enzymes

Programm/Programme

• H2020-EU.1.1. - EXCELLENT SCIENCE - European Research Council (ERC) Main Programme

Thema/Themen

• ERC-2016-STG - ERC Starting Grant

Aufforderung zur Vorschlagseinreichung

ERC-2016-STG

Finanzierungsplan

ERC-STG - Starting Grant

Gastgebende Einrichtung

UPPSALA UNIVERSITET

• Netto-EU-Beitrag: € 1 494 880,00
• Adresse: VON KRAEMERS ALLE 4 ; 751 05 Uppsala ; Schweden
• Region: Östra Sverige › Östra Mellansverige › Uppsala län
• Aktivitätstyp: Higher or Secondary Education Establishments
• Gesamtkosten: € 1 494 880,00

Begünstigte (1)

UPPSALA UNIVERSITET

Schweden

• Netto-EU-Beitrag: € 1 494 880,00
• Adresse: VON KRAEMERS ALLE 4 ; 751 05 Uppsala
• Region: Östra Sverige › Östra Mellansverige › Uppsala län
• Aktivitätstyp: Higher or Secondary Education Establishments
• Gesamtkosten: € 1 494 880,00