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Nanoscale live-cell activity sensing using smart probes and imaging

Objective

Fluorescence microscopy is the tool of choice for live-cell imaging. Its usefulness has been further enhanced by the availability of genetically-encoded biosensors, which enable the visualisation of when and where a certain activity arises. In addition, the development of diffraction-unlimited imaging has dramatically improved the spatial resolution of fluorescence imaging. However, these techniques have had difficulty working with biosensors, largely limiting the information to the spatial location of the labels.
This project seeks to develop diffraction-unlimited imaging of biosensors, creating activity maps with a diffraction-unlimited spatial resolution in living systems. I propose to meet this challenge using a two-pronged approach, focusing both on the development of labels and sensors as well as new imaging tools and strategies. Based on existing scaffolds, we will develop sensors that display strong photochromism, providing reversible fluorescence dynamics intrinsically suited to superresolution imaging. In tandem, we will develop imaging strategies that focus on robustness and work well in living systems, in exchange for a spatial resolution of a 50 to 70 nm and a temporal resolution of a few seconds or less.
We will use these developments in the study of the nanoscale spatiotemporal regulation of G-protein-coupled receptor (GCPR) signalling in living systems. By extending sub-diffraction imaging to the molecular environment, this project will contribute new insights into long-standing research questions that directly involve the health and well-being of all of us, while also enabling exciting prospects for further research.
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Host institution

KATHOLIEKE UNIVERSITEIT LEUVEN

Address

Oude Markt 13
3000 Leuven

Belgium

Activity type

Higher or Secondary Education Establishments

EU Contribution

€ 1 368 250

Beneficiaries (1)

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KATHOLIEKE UNIVERSITEIT LEUVEN

Belgium

EU Contribution

€ 1 368 250

Project information

Grant agreement ID: 714688

Status

Ongoing project

  • Start date

    1 February 2017

  • End date

    31 January 2022

Funded under:

H2020-EU.1.1.

  • Overall budget:

    € 1 368 250

  • EU contribution

    € 1 368 250

Hosted by:

KATHOLIEKE UNIVERSITEIT LEUVEN

Belgium