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Model-Based Construction And Optimisation Of Versatile Chassis Yeast Strains For Production Of Valuable Lipid And Aromatic Compounds

Objective

CHASSY will unlock the full potential of the yeasts Saccharomyces cerevisiae, Yarrowia lipolytica and Kluyveromyces marxianus as cell factories for production of high value compounds which have applications in the cosmetic, nutraceutical and white biotechnology sectors. Current cell factory strains for these classes of product are restricted to proof-of-principle levels because of limited precursor supply, poor product tolerance and lack of versatility. CHASSY addresses these challenges by redesigning metabolic circuits and expanding the host range to include the oleaginous yeast, Y. lipolytica and the thermotolerant yeast, K. marxianus. The systems biology approach will integrate model-based design, construction and analysis of yeast strains, resulting in reconfigured metabolic networks optimised for the production of lipid and aromatic molecules. Construction of the chassis strains, using new and existing synthetic biology tools, will be directed by knowledge derived from a thorough systems biology comparison of the three yeast species, conducted using integrative data analysis and genome scale metabolic models. The chassis strains will be used to build cell factories to produce three specific high value products: the oleochemicals, Docosanol and Octanoic acid; and the aromatic molecule, Amorfrutin 1. These new cell factory strains will be evaluated under industrial conditions to produce data that will further improve the chassis platforms. The major outcomes of this project will be (1) a new set of chassis yeast strains that are widely applicable for development of industrial cell factories; (2) the knowledge and technology to readily build and evaluate new chassis tailored to specific applications; (3) prototype cell factory strains producing three high value metabolites for commercial exploitation; (4) a dissemination and exploitation strategy to ensure that European SMEs benefit from the knowledge base, platform chassis and resources generated in CHASSY.

Coordinator

UNIVERSITY COLLEGE CORK - NATIONAL UNIVERSITY OF IRELAND, CORK

Address

Western Road
T12 Yn60 Cork

Ireland

Activity type

Higher or Secondary Education Establishments

EU Contribution

€ 1 216 232,49

Participants (9)

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TECHNISCHE UNIVERSITEIT DELFT

Netherlands

EU Contribution

€ 950 091,25

CHALMERS TEKNISKA HOEGSKOLA AB

Sweden

EU Contribution

€ 652 135

INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE

France

EU Contribution

€ 747 595

JOHANN WOLFGANG GOETHE-UNIVERSITATFRANKFURT AM MAIN

Germany

EU Contribution

€ 535 500

EVOLVA AG

Switzerland

BIOPETROLIA AB

Sweden

EU Contribution

€ 702 200

NOVA-INSTITUT FUR POLITISCHE UND OKOLOGISCHE INNOVATION GMBH

Germany

EU Contribution

€ 251 000

UCC ACADEMY DESIGNATED ACTIVITY COMPANY

Ireland

EU Contribution

€ 212 500

CLARIANT PRODUKTE (DEUTSCHLAND) GMBH

Germany

EU Contribution

€ 367 356,26

Project information

Grant agreement ID: 720824

Status

Ongoing project

  • Start date

    1 December 2016

  • End date

    30 November 2020

Funded under:

H2020-EU.2.1.4.

  • Overall budget:

    € 5 634 610

  • EU contribution

    € 5 634 610

Coordinated by:

UNIVERSITY COLLEGE CORK - NATIONAL UNIVERSITY OF IRELAND, CORK

Ireland