Exploring the Impact of Pesticide on the 3D Metabolome and the Microbiota from the Whole Plant to the Cell Scale

Projektinformationen

3D-Plant2Cells

ID Finanzhilfevereinbarung: 704786

DOI 10.3030/704786

Das Projekt endete am 5 April 2018

EK-Unterschriftsdatum 7 März 2016

Startdatum 1 Januar 2017

Enddatum 31 Dezember 2019

Finanziert unter

• EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions

Gesamtkosten € 246 668,40

EU-Beitrag € 246 668,40

Koordiniert durch

CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS
France

Ziel

The project 3D-Plant2Cells proposes to explore the impact of the agricultural mode on crops grown for human consumption. We are going to develop innovative interdisciplinary approaches to study the metabolome and the microbiota of plants in three-dimension (3D) at three different scales: the whole plant, the fruit, and the cellular scales. The objective of this study is to get a new insight on the metabolome and microbiota response to pesticide treatment. The hypothesis is that the chemistry and microbiome could change upon the use of conventional cultivation managements. To explore this hypothesis, two groups of plants will be cultivated in growth chamber and one will received pesticide treatments. Then, we will use an innovative interdisplinary approach to explore the entire pool of metabolite (metabolome) and the plant-associated commensal microbes (plant microbiota) on the whole plant surface (plant scale). Samples will be collected on the whole plant and their metabolite content will be profiled by high-resolution tandem-mass spectrometry (HR-MS/MS) and plant-associated micro-organism communities by amplicon sequence analysis. Results of these both inputs will be uploaded into a bioinformatic model representing a 3D topological map of the metabolome/microbiome over the entire organism surface. In a second part of the research project, we will study fruits of the cultivated specimens by 3D mass spectrometry imaging. The goals are to visualize, in a spatially-resolved way, the metabolome/microbiota response to pesticide treatments along with the pesticide distribution in 3D. The metabolome will be investigated at two scales: the fruit and the cell. The fruit scale will be explored by analyzing serial sections using matrix assisted laser desorption ionization (MALDI) or nanodesorption electrospray ionization (nanoDESI) .... The entire project will be an important contribution to assess the impact of pesticide on plant metabolome/microbiome and on food safety.

Wissenschaftliches Gebiet

• natural sciences › computer and information sciences › software
• natural sciences › earth and related environmental sciences › physical geography › cartography
• agricultural sciences › agriculture, forestry, and fisheries › agriculture › horticulture › fruit growing
• natural sciences › chemical sciences › analytical chemistry › mass spectrometry
• natural sciences › biological sciences › microbiology

Schlüsselbegriffe

• 3D mass spectrometry imaging
• tandem mass spectrometry
• molecular networking
• microbiomics

Programm/Programme

• H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions Main Programme
• H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility

Thema/Themen

• MSCA-IF-2015-GF - Marie Skłodowska-Curie Individual Fellowships (IF-GF)

Aufforderung zur Vorschlagseinreichung

H2020-MSCA-IF-2015

Finanzierungsplan

MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)

Koordinator

CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS

• Netto-EU-Beitrag: € 246 668,40
• Adresse: RUE MICHEL ANGE 3 ; 75794 Paris ; Frankreich
• Region: Ile-de-France › Ile-de-France › Paris
• Aktivitätstyp: Research Organisations
• Gesamtkosten: € 246 668,40

Partner (1)

Partner

Partnerorganisationen tragen zur Umsetzung der Aktion bei, unterzeichnen jedoch nicht die Finanzhilfevereinbarung.

THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Vereinigte Staaten

• Netto-EU-Beitrag: € 0,00
• Adresse: FRANKLIN STREET 1111 12 FLOOR ; 94607 OAKLAND CA
• Aktivitätstyp: Higher or Secondary Education Establishments
• Gesamtkosten: € 160 130,40