Objetivo Essential subunits of the mitochondrial respiratory chain, which generates the majority of energy in eukaryotic cells, are encoded in the mitochondrial genome (mtDNA) that is present in hundreds of copies in every cell. Mutations within mtDNA have been identified as the cause for a multitude of human diseases and have been tightly linked to the ageing process and altered stem cell homeostasis. Accordingly, to ensure organismal health, good copies of mtDNA have to be faithfully inherited during cell division, their integrity needs to be maintained over generations and they need to be distributed throughout the mitochondrial network to provide all mitochondrial segments with mtDNA encoded proteins. Astonishingly, it remains poorly understood how cells accomplish these fundamental tasks.Through the development of a novel system that for the first time allowed minimally invasive tracking of mtDNA in living cells, we have gained unique insights into the cellular principles that govern distribution and inheritance of mtDNA and the maintenance of its integrity. This work paved the way to understand the molecular mechanisms that underlie these processes and provides the tools required to elucidate them. We will build on this work and combine cutting-edge microscopy and next generation sequencing with biochemical and genetic approaches to identify and characterize the machineries responsible for (1) mtDNA inheritance and distribution and (2) mtDNA quality control. While these first two aims will exploit the unique experimental advantages of S. cerevisiae, our ultimate goal is (3) to transfer our findings to higher eukaryotes through the development of a mammalian mtDNA imaging system.This powerful multipronged approach will mechanistically unravel mtDNA dynamics and quality control and will thus provide the necessary basis to understand diseases where these processes are dysregulated. Ámbito científico natural sciencesbiological sciencesgeneticsDNAnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsnatural sciencesphysical sciencesopticsmicroscopynatural sciencesbiological sciencesgeneticsmutationnatural sciencesbiological sciencesgeneticsgenomes Programa(s) H2020-EU.1.1. - EXCELLENT SCIENCE - European Research Council (ERC) Main Programme Tema(s) ERC-2016-STG - ERC Starting Grant Convocatoria de propuestas ERC-2016-STG Consulte otros proyectos de esta convocatoria Régimen de financiación ERC-STG - Starting Grant Institución de acogida LUDWIG-MAXIMILIANS-UNIVERSITAET MUENCHEN Aportación neta de la UEn € 1 851 834,00 Dirección GESCHWISTER SCHOLL PLATZ 1 80539 Muenchen Alemania Ver en el mapa Región Bayern Oberbayern München, Kreisfreie Stadt Tipo de actividad Higher or Secondary Education Establishments Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Participación en los programas de I+D de la UE Opens in new window Red de colaboración de HORIZON Opens in new window Coste total € 1 851 834,00 Beneficiarios (1) Ordenar alfabéticamente Ordenar por aportación neta de la UE Ampliar todo Contraer todo LUDWIG-MAXIMILIANS-UNIVERSITAET MUENCHEN Alemania Aportación neta de la UEn € 1 851 834,00 Dirección GESCHWISTER SCHOLL PLATZ 1 80539 Muenchen Ver en el mapa Región Bayern Oberbayern München, Kreisfreie Stadt Tipo de actividad Higher or Secondary Education Establishments Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Participación en los programas de I+D de la UE Opens in new window Red de colaboración de HORIZON Opens in new window Coste total € 1 851 834,00
