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Development of high-resolution, high-throughput microscopy

Objective

Current 3D microscopy methods are limited in speed due to the need to scan the sample in the optical axis. Multifocus microscopy - or MFM- is a method that allows for the instant acquisition of a 3D volume without scanning, thus increasing acquisition speed by a factor of 10-100. MFM based on diffractive optics allows for easy optimization of the total volume acquired and the sampling rate. In our ERC-StG project, we developed an important expertise in the design and construction of diffractive elements key to MFM and invented a novel super-resolution imaging method based on this technology (patent pending). In this project, we propose to further improve the conception and fabrication methods of MFM’s diffractive elements to considerably improve acquisition speed and resolution. In addition, we will employ these technologies in conjunction with valve-based microfluidics to develop and validate a microscopy-based high-resolution, high-throughput cell profiler (Hi2M). Hi2M will allow for the 3D acquisition of complete cell volumes in <10 ms, enabling the high-resolution, multi-color imaging of millions of cells/hour without the need for cell adhesion. Because of these important advantages over existing microscopies, we anticipate that the technologies developed in InstantScope should have a large impact in many fields of cell biology.
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Host institution

INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE

Address

Rue De Tolbiac 101
75654 Paris

France

Activity type

Research Organisations

EU Contribution

€ 149 788

Beneficiaries (1)

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INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE

France

EU Contribution

€ 149 788

Project information

Grant agreement ID: 737560

Status

Closed project

  • Start date

    1 March 2017

  • End date

    31 August 2018

Funded under:

H2020-EU.1.1.

  • Overall budget:

    € 149 788

  • EU contribution

    € 149 788

Hosted by:

INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE

France