Objetivo The inner working of many fundamental biological nanomachines implies many stochastic changes in conformation and molecular interactions. These changes are reflected as variability in the activity of the same molecule over time, and in heterogeneous activities between different molecules. By the direct observation at the molecular scale of these nanomachines activity, single-molecule fluorescence techniques have gained access to this stochastic information, which is otherwise missed by bulk techniques but is essential for their understanding. However, the widespread use of these techniques in many biological systems has been hampered by the low working concentration (nM) determined by the diffraction limit of light, and current nanophotonic solutions to this problem are technically too demanding. Here, we propose self-assembled DNA origami nanoantennas as nanophotonic platforms aiming to break this concentration barrier by means of fluorescence signal enhancing and reduction of the observation volume. The versatility of DNA origami structures, biocompatibility and ability for site-directed immobilization of biomolecules, make them perfectly suited to perform complex biological assays. In the presented action we aim to achieve single-molecule fluorescence DNA sequencing using DNA origami nanoantennas to probe the potential of these platforms to perform complex bioassays, in the high concentration regime and demanding multiplexing. Moreover, since they avoid the fabrication and instrumental challenges related to other nanophotonic devices, self-assembly DNA origami nanoantennas are amenable, easy to handle and friendly technology to the biological experimenter, and thus we expect to boost their use in biology. Besides, the fulfilment of this action will provide the fellow with a complete formative training that would boost his future scientific career, and will generate also a new DNA-sequencing technology that will impact positively European scientific excellence. Ámbito científico engineering and technologymaterials engineeringcolorsnatural sciencesbiological sciencesgeneticsDNAnatural sciencesbiological sciencesbiochemistrybiomoleculesengineering and technologynanotechnologynano-materials Programa(s) H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions Main Programme H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility Tema(s) MSCA-IF-2016 - Individual Fellowships Convocatoria de propuestas H2020-MSCA-IF-2016 Consulte otros proyectos de esta convocatoria Régimen de financiación MSCA-IF-EF-ST - Standard EF Coordinador LUDWIG-MAXIMILIANS-UNIVERSITAET MUENCHEN Aportación neta de la UEn € 86 374,60 Dirección GESCHWISTER SCHOLL PLATZ 1 80539 Muenchen Alemania Ver en el mapa Región Bayern Oberbayern München, Kreisfreie Stadt Tipo de actividad Higher or Secondary Education Establishments Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Participación en los programas de I+D de la UE Opens in new window Red de colaboración de HORIZON Opens in new window Coste total € 86 374,60 Participantes (1) Ordenar alfabéticamente Ordenar por aportación neta de la UE Ampliar todo Contraer todo TECHNISCHE UNIVERSITAET BRAUNSCHWEIG La participación finalizó Alemania Aportación neta de la UEn € 73 086,20 Dirección UNIVERSITAETSPLATZ 2 38106 Braunschweig Ver en el mapa Región Niedersachsen Braunschweig Braunschweig, Kreisfreie Stadt Tipo de actividad Higher or Secondary Education Establishments Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Participación en los programas de I+D de la UE Opens in new window Red de colaboración de HORIZON Opens in new window Coste total € 73 086,20
