Obiettivo Introduction-state of art of the problem: The main goal of the present proposal is to devise a chemical sensor capable of gluten detection in food. Gluten is the allergen that triggers autoimmuno reactions in people suffering of celiac disease (CD). CD is an autoimmune disorder, characterized a variety of genetic assets, which is estimated to affect the 1-2 % of the European population with direct costs on the healthcare estimated as 3 bn Euros/year. According to the state of the current knowledge gluten-free diet is mandatory in CD. Food shall contain less than 20 mg/kg gluten of to be considered as “gluten-free” and to be eaten safely. Scientific part of the project: To face the problem of CD we are coming with the idea to devise a chemical sensor capable of fast gluten detection in food. To complete this task, we will design a recognition unit using the technology of molecularly imprinted polymers (MIPs), which allows to produce synthetic receptors by a template assisted synthesis. Being gluten a mix of proteins (prolamins) the design of the MIPs will be rationalized by the aid of the epitope imprinting methodology, that complements protein 3D- and linear sequence-databases, so to find distinctive portions of the protein to be used as templates. The next step is an electrode-MIP preparation based on thiophene monomers that will proceed through a foregoing rational design of the monomers selection by computational methods. In the third step we will focus on the study of the performance of the sensor both in model solutions and in real samples. Finally, validation on true samples will be performed by comparing our results to independent analytical methods.Expected outcomes and impact of the work: As result of the proposed project, the laboratory model of the working gluten chemosensor will be developed. Such model will be useful to devise a prototype of a gluten detector ready for commercialization. Campo scientifico natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsnatural scienceschemical sciencespolymer sciencesmedical and health scienceshealth sciencesnutritionengineering and technologyelectrical engineering, electronic engineering, information engineeringelectronic engineeringsensorsmedical and health sciencesclinical medicinegastroenterology Parole chiave Allergen celiac disease chemical sensor diet epitope food gluten health molecularly imprinted polymer (MIP) peptide protein Programma(i) H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions Main Programme H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility Argomento(i) MSCA-IF-2016 - Individual Fellowships Invito a presentare proposte H2020-MSCA-IF-2016 Vedi altri progetti per questo bando Meccanismo di finanziamento MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF) Coordinatore UNIVERSITA DEGLI STUDI DI VERONA Contribution nette de l'UE € 180 277,20 Indirizzo VIA DELL ARTIGLIERE 8 37129 Verona Italia Mostra sulla mappa Regione Nord-Est Veneto Verona Tipo di attività Higher or Secondary Education Establishments Collegamenti Contatta l’organizzazione Opens in new window Sito web Opens in new window Partecipazione a programmi di R&I dell'UE Opens in new window Rete di collaborazione HORIZON Opens in new window Costo totale € 180 277,20