Objective There is an urgent need for fast and accurate diagnostic tests in the current Ebola crisis. The rapid diagnosis of EVD during early and late stage of infection is a decisive step for risk assessment and for guidance to physicians to take the necessary decisions to limit the spread of the infection, and to safely nurse the infected patients. While fast and easy-to-use tests usually rely on immuno-diagnostic approaches, they typically lack high sensitivity and specificity. The gold standard for accurate diagnostics, (Real-Time) PCR, however, is limited in terms of processing time, high costs, and in most cases also in terms of ease of use and the need of special laboratory facilities.The aim of this project is to provide an ultra-fast and accurate molecular diagnostic instrument that can perform a molecular Ebola test within 10-15 minutes. Patients, suspected of Ebola Virus Disease (EBD), due to fever and/or other clinical signs, could be rapidly and accurately tested at the Point-of-Care by withdrawal of blood, or less invasive fluids, such as saliva or urine.The core technology of this proposal is based on laser-heated nanoparticles (Laser PCR), overcoming the time-limiting step of heating and cooling the reaction sample in conventional PCR allowing for drastically reduced turn-around times. The project partners (GNA, INMI, MUB, EMG) have the competence to integrate sample preparation with virus-binding magnetic particles, ultra-fast molecular diagnostics with Laser PCR, test the system in a biosafety level 4 laboratory in Rome, Italy, and thoroughly conduct field testing in a medical care centre in West Africa (Sierra Leone).A tentative Ebola Laser PCR assay has already been set up, detecting 10 target copies of synthetic DNA oligos, corresponding to the Ebola genome sequence, in less than 12 minutes. The FILODIAG project will deliver a potentially multiplexed diagnostic system fast enough for point-of need testing of incoming patients at low-resource Fields of science natural sciencesbiological sciencesmicrobiologyvirologymedical and health scienceshealth sciencesinfectious diseasesRNA virusesebolaengineering and technologynanotechnologynano-materialsnatural sciencesbiological sciencesgeneticsgenomesnatural sciencesphysical sciencesopticslaser physics Programme(s) H2020-EU.3.1. - SOCIETAL CHALLENGES - Health, demographic change and well-being Main Programme H2020-EU.3.1.7. - Innovative Medicines Initiative 2 (IMI2) Topic(s) IMI2-2014-02-05 - Rapid diagnostic tests Call for proposal H2020-JTI-IMI2-2014-02-single-stage See other projects for this call Funding Scheme IMI2-RIA - Research and Innovation action Coordinator HP HEALTH SOLUTIONS GERMANY GMBH Net EU contribution € 1 461 750,00 Address Am Klopferspitz 19 82152 Martinsried Germany See on map SME The organization defined itself as SME (small and medium-sized enterprise) at the time the Grant Agreement was signed. Yes Region Bayern Oberbayern München, Landkreis Activity type Private for-profit entities (excluding Higher or Secondary Education Establishments) Links Contact the organisation Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Total cost € 1 461 750,00 Participants (3) Sort alphabetically Sort by Net EU contribution Expand all Collapse all MENDELOVA UNIVERZITA V BRNE Czechia Net EU contribution € 381 750,00 Address ZEMEDELSKA 1/1665 613 00 Brno Sever See on map Region Česko Jihovýchod Jihomoravský kraj Activity type Higher or Secondary Education Establishments Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Total cost € 381 750,00 ISTITUTO NAZIONALE PER LE MALATTIE INFETTIVE LAZZARO SPALLANZANI-ISTITUTO DI RICOVERO E CURA A CARATTERE SCIENTIFICO Italy Net EU contribution € 404 105,00 Address Via Portuense, 292 00149 Roma See on map Region Centro (IT) Lazio Roma Activity type Research Organisations Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Total cost € 404 105,00 EMERGENCY LIFE SUPPORT FOR CIVILIAN WAR VICTIMS ONG ONLUS Italy Net EU contribution € 12 500,00 Address VIA SANTA CROCE 19 20122 Milano See on map Region Nord-Ovest Lombardia Milano Activity type Other Links Contact the organisation Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Total cost € 12 500,00